Literature DB >> 31758462

Assays to Evaluate Toxoplasma-Macrophage Interactions.

Debanjan Mukhopadhyay1, Jeroen P J Saeij2.   

Abstract

The obligate intracellular protozoan parasite Toxoplasma gondii can infect any nucleated cell from a warm-blooded host. However, its interaction with host macrophages plays a critical role in shaping the immune response during infection. Therefore, assessing Toxoplasma-macrophage interactions at a cellular level is important. In this chapter, we describe assays that can be used to characterize Toxoplasma-macrophage interactions. These assays can also be used to evaluate other host-pathogen interactions. We describe multiplex approaches for measuring arginase activity, indoleamine 2,3 dioxygenase activity, cell death, and parasite growth during Toxoplasma-macrophage interactions. These assays can be used to compare how different Toxoplasma strains differ in their interaction with macrophages, and we describe how to properly assess Toxoplasma strain differences in Toxoplasma-macrophage interactions.

Entities:  

Keywords:  Arginase; GRA15; IDO; Inflammasome; LDH; MTS; Macrophage; Macrophage polarization; ROP16; Toxoplasma

Year:  2020        PMID： 31758462      PMCID： PMC7048168          DOI： 10.1007/978-1-4939-9857-9_19

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  22 in total

1.  Toxoplasma polymorphic effectors determine macrophage polarization and intestinal inflammation.

Authors:  Kirk D C Jensen; Yiding Wang; Elia D Tait Wojno; Anjali J Shastri; Kenneth Hu; Lara Cornel; Erwan Boedec; Yi-Ching Ong; Yueh-hsiu Chien; Christopher A Hunter; John C Boothroyd; Jeroen P J Saeij
Journal:  Cell Host Microbe       Date:  2011-06-16       Impact factor: 21.023

2.  Inhibition of growth of Toxoplasma gondii in cultured fibroblasts by human recombinant gamma interferon.

Authors:  E R Pfefferkorn; P M Guyre
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

3.  Use of an aqueous soluble tetrazolium/formazan assay to measure viability and proliferation of lymphokine-dependent cell lines.

Authors:  T M Buttke; J A McCubrey; T C Owen
Journal:  J Immunol Methods       Date:  1993-01-04       Impact factor: 2.303

4.  Transcriptional and Linkage Analyses Identify Loci that Mediate the Differential Macrophage Response to Inflammatory Stimuli and Infection.

Authors:  Musa A Hassan; Kirk D Jensen; Vincent Butty; Kenneth Hu; Erwan Boedec; Pjotr Prins; Jeroen P J Saeij
Journal:  PLoS Genet       Date:  2015-10-28       Impact factor: 5.917

Review 5.  The human immune response to Toxoplasma: Autophagy versus cell death.

Authors:  Shruthi Krishnamurthy; Eleni K Konstantinou; Lucy H Young; Daniel A Gold; Jeroen P J Saeij
Journal:  PLoS Pathog       Date:  2017-03-09       Impact factor: 6.823

Review 6.  Exposing Toxoplasma gondii hiding inside the vacuole: a role for GBPs, autophagy and host cell death.

Authors:  Jeroen P Saeij; Eva-Maria Frickel
Journal:  Curr Opin Microbiol       Date:  2017-11-12       Impact factor: 7.934

7.  Macrophage activation and polarization: nomenclature and experimental guidelines.

Authors:  Peter J Murray; Judith E Allen; Subhra K Biswas; Edward A Fisher; Derek W Gilroy; Sergij Goerdt; Siamon Gordon; John A Hamilton; Lionel B Ivashkiv; Toby Lawrence; Massimo Locati; Alberto Mantovani; Fernando O Martinez; Jean-Louis Mege; David M Mosser; Gioacchino Natoli; Jeroen P Saeij; Joachim L Schultze; Kari Ann Shirey; Antonio Sica; Jill Suttles; Irina Udalova; Jo A van Ginderachter; Stefanie N Vogel; Thomas A Wynn
Journal:  Immunity       Date:  2014-07-17       Impact factor: 31.745

8.  A Toxoplasma dense granule protein, GRA24, modulates the early immune response to infection by promoting a direct and sustained host p38 MAPK activation.

Authors:  Laurence Braun; Marie-Pierre Brenier-Pinchart; Manickam Yogavel; Aurélie Curt-Varesano; Rose-Laurence Curt-Bertini; Tahir Hussain; Sylvie Kieffer-Jaquinod; Yohann Coute; Hervé Pelloux; Isabelle Tardieux; Amit Sharma; Hassan Belrhali; Alexandre Bougdour; Mohamed-Ali Hakimi
Journal:  J Exp Med       Date:  2013-09-16       Impact factor: 14.307

9.  Inflammasome sensor NLRP1 controls rat macrophage susceptibility to Toxoplasma gondii.

Authors:  Kimberly M Cirelli; Gezahegn Gorfu; Musa A Hassan; Morton Printz; Devorah Crown; Stephen H Leppla; Michael E Grigg; Jeroen P J Saeij; Mahtab Moayeri
Journal:  PLoS Pathog       Date:  2014-03-13       Impact factor: 6.823

10.  Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes.

Authors:  Hernan Lorenzi; Asis Khan; Michael S Behnke; Sivaranjani Namasivayam; Lakshmipuram S Swapna; Michalis Hadjithomas; Svetlana Karamycheva; Deborah Pinney; Brian P Brunk; James W Ajioka; Daniel Ajzenberg; John C Boothroyd; Jon P Boyle; Marie L Dardé; Maria A Diaz-Miranda; Jitender P Dubey; Heather M Fritz; Solange M Gennari; Brian D Gregory; Kami Kim; Jeroen P J Saeij; Chunlei Su; Michael W White; Xing-Quan Zhu; Daniel K Howe; Benjamin M Rosenthal; Michael E Grigg; John Parkinson; Liang Liu; Jessica C Kissinger; David S Roos; L David Sibley
Journal:  Nat Commun       Date:  2016-01-07       Impact factor: 14.919
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