Luke Potts1, Casie Phillips2, Munok Hwang3, Samuel Fulcher4,5, Hosoon Choi2,5. 1. Department of Ophthalmology and Surgery, Scott and White Eye Institute, Temple, Texas 76508, USA. 2. Central Texas Veterans Research Foundation, Temple, Texas 76504, USA. 3. Department of Biomedical Informatics, University of Texas Health Science Center at Houston, Houston, Texas 77030, USA. 4. Department of Surgery, Central Texas Veterans Health Care System, Temple, Texas 76054, USA. 5. Department of Medicine, College of Medicine, Texas A&M Health Science Center, Bryan, TX 77807, USA.
Abstract
AIM: To study the effect of renalase peptide, RP-220, on cell viability of human corneal epithelial cells after alkali insult. METHODS: A dose-response relationship between cell viability and exposure to NaOH solution were characterized using cultured human corneal epithelial cells. Viability of corneal epithelial cells was determined using commercially available MTT and CyQUANT® assays. RESULTS: At a concentration of 6 mmol/L, insult with NaOH leads to reduced corneal epithelial cell viability by approximately 30%. This reduced viability was prevented by treating the cells after initial insult with the 20-amino acid renalase derived peptide (RP-220). CONCLUSION: RP-220 has a pro-survival role for RP-220 following alkaline insult to corneal epithelial cells. International Journal of Ophthalmology Press.
AIM: To study the effect of renalase peptide, RP-220, on cell viability of human corneal epithelial cells after alkali insult. METHODS: A dose-response relationship between cell viability and exposure to NaOH solution were characterized using cultured human corneal epithelial cells. Viability of corneal epithelial cells was determined using commercially available MTT and CyQUANT® assays. RESULTS: At a concentration of 6 mmol/L, insult with NaOH leads to reduced corneal epithelial cell viability by approximately 30%. This reduced viability was prevented by treating the cells after initial insult with the 20-amino acidrenalase derived peptide (RP-220). CONCLUSION: RP-220 has a pro-survival role for RP-220 following alkaline insult to corneal epithelial cells. International Journal of Ophthalmology Press.
Entities:
Keywords:
RP-220; corneal alkali injury; human corneal epithelial cells; renalase
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