Literature DB >> 31741062

Stable transformation of unicellular green alga Coccomyxa subellipsoidea C-169 via electroporation.

Kinga Kania1, Maksymilian Zienkiewicz1, Anna Drożak2.   

Abstract

In this study, we have shown the applicability of electroporation and hygromycin B as a convenient selectable marker for stable nuclear transformation of Coccomyxa subellipsoidea C-169. Since it is the first sequenced eukaryotic microorganism from polar environment, this offers unique opportunities to study adaptation mechanisms to cold.

Entities:  

Keywords:  Coccomyxa subellipsoidea C-169; Electroporation; Electrotransformation; Hygromycin B resistance; Stable genome transformation

Year:  2019        PMID： 31741062     DOI： 10.1007/s00709-019-01447-2

Source DB:  PubMed          Journal:  Protoplasma        ISSN： 0033-183X            Impact factor:   3.356


  10 in total

Review 1.  Selection, breeding and engineering of microalgae for bioenergy and biofuel production.

Authors:  Anthony W D Larkum; Ian L Ross; Olaf Kruse; Ben Hankamer
Journal:  Trends Biotechnol       Date:  2011-12-16       Impact factor: 19.536

2.  Enhanced chemiluminescence (ECL) for routine immunoblotting: An inexpensive alternative to commercially available kits.

Authors:  Dolores D Mruk; C Yan Cheng
Journal:  Spermatogenesis       Date:  2011-04

3.  High-frequency nuclear transformation of Chlamydomonas reinhardtii.

Authors:  K L Kindle
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

4.  High-efficiency transformation of Chlamydomonas reinhardtii by electroporation.

Authors:  K Shimogawara; S Fujiwara; A Grossman; H Usuda
Journal:  Genetics       Date:  1998-04       Impact factor: 4.562

5.  Photodegradation products of chloramphenicol in aqueous solution.

Authors:  I K Shih
Journal:  J Pharm Sci       Date:  1971-12       Impact factor: 3.534

6.  High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.

Authors:  Oliver Kilian; Christina S E Benemann; Krishna K Niyogi; Bertrand Vick
Journal:  Proc Natl Acad Sci U S A       Date:  2011-11-28       Impact factor: 11.205

7.  Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation.

Authors:  L E Brown; S L Sprecher; L R Keller
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

8.  Comparison of hCMV immediate early and CaMV 35S promoters in both plant and human cells.

Authors:  Josef Vlasák; Michal Smahel; Adam Pavlík; Daniela Pavingerová; Jindrich Bríza
Journal:  J Biotechnol       Date:  2003-08-15       Impact factor: 3.307

9.  The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation.

Authors:  Guillaume Blanc; Irina Agarkova; Jane Grimwood; Alan Kuo; Andrew Brueggeman; David D Dunigan; James Gurnon; Istvan Ladunga; Erika Lindquist; Susan Lucas; Jasmyn Pangilinan; Thomas Pröschold; Asaf Salamov; Jeremy Schmutz; Donald Weeks; Takashi Yamada; Alexandre Lomsadze; Mark Borodovsky; Jean-Michel Claverie; Igor V Grigoriev; James L Van Etten
Journal:  Genome Biol       Date:  2012-05-25       Impact factor: 13.583

10.  Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

Authors:  Roman J Szczesny; Katarzyna Kowalska; Kamila Klosowska-Kosicka; Aleksander Chlebowski; Ewelina P Owczarek; Zbigniew Warkocki; Tomasz M Kulinski; Dorota Adamska; Kamila Affek; Agata Jedroszkowiak; Anna V Kotrys; Rafal Tomecki; Pawel S Krawczyk; Lukasz S Borowski; Andrzej Dziembowski
Journal:  PLoS One       Date:  2018-03-28       Impact factor: 3.240
  10 in total
  2 in total

1.  The effect of temperature conditions during growth on the transformation frequency of Coccomyxa subellipsoidea C-169 obtained by electroporation.

Authors:  Kinga Kania; Anna Levytska; Anna Drożak; Borkowski Andrzej; Działak Paweł; Maksymilian Zienkiewicz
Journal:  Biochem Biophys Rep       Date:  2022-02-07

Review 2.  Plant-microbe interactions that have impacted plant terrestrializations.

Authors:  Camille Puginier; Jean Keller; Pierre-Marc Delaux
Journal:  Plant Physiol       Date:  2022-08-29       Impact factor: 8.005
  2 in total

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