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Literature DB >> 31723075

A Survey of Genome Editing Activity for 16 Cas12a Orthologs.

Bernd Zetsche^1,2,3,4,5, Omar O Abudayyeh^1,2,3,4,6, Jonathan S Gootenberg^1,2,3,4,7, David A Scott^1,2,3,4, Feng Zhang^1,2,3,4.

Abstract

The class 2 CRISPR-Cas endonuclease Cas12a (previously known as Cpf1) offers several advantages over Cas9, including the ability to process its own array and the requirement for just a single RNA guide. These attributes make Cas12a promising for many genome engineering applications. To further expand the suite of Cas12a tools available, we tested 16 Cas12a orthologs for activity in eukaryotic cells. Four of these new enzymes demonstrated targeted activity, one of which, from Moraxella bovoculi AAX11_00205 (Mb3Cas12a), exhibited robust indel formation. We also showed that Mb3Cas12a displays some tolerance for a shortened PAM (TTN versus the canonical Cas12a PAM TTTV). The addition of these enzymes to the genome editing toolbox will further expand the utility of this powerful technology.

Entities: CellLine Chemical Disease Gene Species

Keywords: CRISPR-Cas; gene therapy; genome editing

Mesh：

Substances：

Year: 2019 PMID： 31723075 PMCID： PMC7220826 DOI： 10.2302/kjm.2019-0009-OA

Source DB: PubMed Journal: Keio J Med ISSN： 0022-9717

18 in total

1. Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System.

Authors: Mugui Wang; Yanfei Mao; Yuming Lu; Xiaoping Tao; Jian-Kang Zhu
Journal: Mol Plant Date: 2017-03-16 Impact factor: 13.164

2. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites.

Authors: Sanyuan Ma; Yue Liu; Yuanyuan Liu; Jiasong Chang; Tong Zhang; Xiaogang Wang; Run Shi; Wei Lu; Xiaojuan Xia; Ping Zhao; Qingyou Xia
Journal: Insect Biochem Mol Biol Date: 2017-02-09 Impact factor: 4.714

3. The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA.

Authors: Ines Fonfara; Hagen Richter; Majda Bratovič; Anaïs Le Rhun; Emmanuelle Charpentier
Journal: Nature Date: 2016-04-20 Impact factor: 49.962

4. Generation of knockout mice by Cpf1-mediated gene targeting.

Authors: Yongsub Kim; Seung-A Cheong; Jong Geol Lee; Sang-Wook Lee; Myeong Sup Lee; In-Jeoung Baek; Young Hoon Sung
Journal: Nat Biotechnol Date: 2016-06-06 Impact factor: 54.908

5. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.

Authors: Jonathan S Gootenberg; Omar O Abudayyeh; Max J Kellner; Julia Joung; James J Collins; Feng Zhang
Journal: Science Date: 2018-02-15 Impact factor: 47.728

6. RNA-guided human genome engineering via Cas9.

Authors: Prashant Mali; Luhan Yang; Kevin M Esvelt; John Aach; Marc Guell; James E DiCarlo; Julie E Norville; George M Church
Journal: Science Date: 2013-01-03 Impact factor: 47.728

7. DNA targeting specificity of RNA-guided Cas9 nucleases.

Authors: Patrick D Hsu; David A Scott; Joshua A Weinstein; F Ann Ran; Silvana Konermann; Vineeta Agarwala; Yinqing Li; Eli J Fine; Xuebing Wu; Ophir Shalem; Thomas J Cradick; Luciano A Marraffini; Gang Bao; Feng Zhang
Journal: Nat Biotechnol Date: 2013-07-21 Impact factor: 54.908

8. Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida.

Authors: Akira Endo; Mikami Masafumi; Hidetaka Kaya; Seiichi Toki
Journal: Sci Rep Date: 2016-12-01 Impact factor: 4.379

9. Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.

Authors: Bernd Zetsche; Matthias Heidenreich; Prarthana Mohanraju; Iana Fedorova; Jeroen Kneppers; Ellen M DeGennaro; Nerges Winblad; Sourav R Choudhury; Omar O Abudayyeh; Jonathan S Gootenberg; Wen Y Wu; David A Scott; Konstantin Severinov; John van der Oost; Feng Zhang
Journal: Nat Biotechnol Date: 2016-12-05 Impact factor: 54.908

10. Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells.

Authors: Benjamin P Kleinstiver; Shengdar Q Tsai; Michelle S Prew; Nhu T Nguyen; Moira M Welch; Jose M Lopez; Zachary R McCaw; Martin J Aryee; J Keith Joung
Journal: Nat Biotechnol Date: 2016-06-27 Impact factor: 54.908

13 in total

1. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition.

Authors: Jin Zhou; Peng Chen; Hongjian Wang; Huan Liu; Yongzheng Li; Youpeng Zhang; Yankang Wu; Chonil Paek; Zaiqiao Sun; Jun Lei; Lei Yin
Journal: Mol Ther Date: 2021-10-20 Impact factor: 11.454

2. Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii.

Authors: Ruslan Vasilev; Natalia Gunitseva; Regina Shebanova; Aleksei Korzhenkov; Anna Vlaskina; Marta Evteeva; Irina Polushkina; Natalia Nikitchina; Stepan Toshchakov; Piotr Kamenski; Maxim Patrushev; Ilya Mazunin
Journal: Int J Mol Sci Date: 2022-08-18 Impact factor: 6.208

A Survey of Genome Editing Activity for 16 Cas12a Orthologs.

1. Multiplex Gene Editing in Rice Using the CRISPR-Cpf1 System.

2. An integrated CRISPR Bombyx mori genome editing system with improved efficiency and expanded target sites.

3. The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA.

4. Generation of knockout mice by Cpf1-mediated gene targeting.

5. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6.

6. RNA-guided human genome engineering via Cas9.

7. DNA targeting specificity of RNA-guided Cas9 nucleases.

8. Efficient targeted mutagenesis of rice and tobacco genomes using Cpf1 from Francisella novicida.

9. Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array.

10. Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells.

1. Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition.

2. Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii.

3. Efficient genome editing by CRISPR-Mb3Cas12a in mice.

4. Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity.

Review 5. Allele-specific genome targeting in the development of precision medicine.

Review 6. CRISPR technologies and the search for the PAM-free nuclease.

7. A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing.

Review 8. The Rise of the CRISPR/Cpf1 System for Efficient Genome Editing in Plants.

9. Novel Type V-A CRISPR Effectors Are Active Nucleases with Expanded Targeting Capabilities.

10. High-Throughput Profiling of Cas12a Orthologues and Engineered Variants for Enhanced Genome Editing Activity.