Decoding the microbiome for the development of translational applications: Overview, challenges and pitfalls.

Recent studies have highlighted the potential of 'translational' microbiome research in addressing real-world challenges pertaining to human health, nutrition and disease. Additionally, outcomes of microbiome research have also positively impacted various aspects pertaining to agricultural productivity, fuel or energy requirements, and stability/preservation of various ecological habitats. Microbiome data is multi-dimensional with various types of data comprising nucleic and protein sequences, metabolites as well as various metadata related to host and or environment. This poses a major challenge for computational analysis and interpretation of data to reach meaningful, reproducible (and replicable) biological conclusions. In this review, we first describe various aspects of microbiomes that make them an attractive tool/target for developing various translational applications. The challenge of deciphering signatures from an information-rich resource like the microbiome is also discussed. Subsequently, we present three case-studies that exemplify the potential of microbiome- based solutions in solving real-world problems. The final part of the review attempts to familiarize readers with the importance of a robust study design and the diligence required during every stage of analysis for achieving solutions with potential translational value.