Literature DB >> 3171478

Molecular cloning of an immunodominant antigen of Onchocerca volvulus.

R Lucius1, N Erondu, A Kern, J E Donelson.   

Abstract

We have cloned and characterized the gene for an immunodominant antigen of O. volvulus that is recognized by the sera of 96% of patients with onchocerciasis. Its 1.2-kb mRNA constitutes 0.3% of adult worm poly(A)+ RNA and its cDNA sequence reveals that it is not a highly conserved structural protein such as actin or tubulin. Similar but not identical genes occur in the genomes of related filarie, Brugia malayi and Dirofilaria immitis. The recombinant antigen has both immunodiagnostic and immunoprophylactic significance.

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Year:  1988        PMID： 3171478      PMCID： PMC2189044          DOI： 10.1084/jem.168.3.1199

Source DB:  PubMed          Journal:  J Exp Med        ISSN： 0022-1007            Impact factor:   14.307


  12 in total

1.  Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase.

Authors:  D B Smith; K M Davern; P G Board; W U Tiu; E G Garcia; G F Mitchell
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

2.  Identification of paramyosin as schistosome antigen recognized by intradermally vaccinated mice.

Authors:  D E Lanar; E J Pearce; S L James; A Sher
Journal:  Science       Date:  1986-10-31       Impact factor: 47.728

3.  Molecular cloning of a protective antigen of schistosomes.

Authors:  J M Balloul; P Sondermeyer; D Dreyer; M Capron; J M Grzych; R J Pierce; D Carvallo; J P Lecocq; A Capron
Journal:  Nature       Date:  1987 Mar 12-18       Impact factor: 49.962

4.  Isolation of living adult Onchocerca volvulus from nodules.

Authors:  H Schulz-Key; E J Albiez; D W Büttner
Journal:  Tropenmed Parasitol       Date:  1977-12

5.  Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors:  P S Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

7.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

8.  Cloning and characterization of two Onchocerca volvulus repeated DNA sequences.

Authors:  F B Perler; M Karam
Journal:  Mol Biochem Parasitol       Date:  1986-11       Impact factor: 1.759

9.  Observations on Onchocerca volvulus in experimentally infected chimpanzees.

Authors:  B O Duke
Journal:  Tropenmed Parasitol       Date:  1980-03

10.  Characterization of an Onchocerca-specific DNA clone from Onchocerca volvulus.

Authors:  J S Shah; M Karam; W F Piessens; D F Wirth
Journal:  Am J Trop Med Hyg       Date:  1987-09       Impact factor: 2.345
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  5 in total

Review 1.  [Molecular biological techniques in the diagnosis of tropical parasitic diseases].

Authors:  R Felleisen; M Q Klinkert
Journal:  Naturwissenschaften       Date:  1992-11

2.  Parasite-specific immunomodulatory functions of filarial cystatin.

Authors:  Peter Schierack; Richard Lucius; Bettina Sonnenburg; Klaus Schilling; Susanne Hartmann
Journal:  Infect Immun       Date:  2003-05       Impact factor: 3.441

3.  Characterizing Reactivity to Onchocerca volvulus Antigens in Multiplex Bead Assays.

Authors:  Karla R Feeser; Vitaliano Cama; Jeffrey W Priest; Elizabeth A Thiele; Ryan E Wiegand; Thomson Lakwo; Sindew M Feleke; Paul T Cantey
Journal:  Am J Trop Med Hyg       Date:  2017-07-19       Impact factor: 2.345

4.  Molecular cloning and characterization of recombinant parasite antigens for immunodiagnosis of onchocerciasis.

Authors:  R Chandrashekar; K Masood; R M Alvarez; A F Ogunrinade; R Lujan; F O Richards; G J Weil
Journal:  J Clin Invest       Date:  1991-11       Impact factor: 14.808

5.  A four-antigen mixture for rapid assessment of Onchocerca volvulus infection.

Authors:  Peter D Burbelo; Hannah P Leahy; Michael J Iadarola; Thomas B Nutman
Journal:  PLoS Negl Trop Dis       Date:  2009-05-19
  5 in total

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