| Literature DB >> 31675496 |
Qiming Zhang1, Yao He2, Nan Luo3, Shashank J Patel4, Yanjie Han1, Ranran Gao1, Madhura Modak5, Sebastian Carotta6, Christian Haslinger7, David Kind7, Gregory W Peet4, Guojie Zhong1, Shuangjia Lu1, Weihua Zhu8, Yilei Mao9, Mengmeng Xiao10, Michael Bergmann11, Xueda Hu1, Sid P Kerkar4, Anne B Vogt12, Stefan Pflanz5, Kang Liu13, Jirun Peng14, Xianwen Ren15, Zemin Zhang16.
Abstract
The immune microenvironment of hepatocellular carcinoma (HCC) is poorly characterized. Combining two single-cell RNA sequencing technologies, we produced transcriptomes of CD45+ immune cells for HCC patients from five immune-relevant sites: tumor, adjacent liver, hepatic lymph node (LN), blood, and ascites. A cluster of LAMP3+ dendritic cells (DCs) appeared to be the mature form of conventional DCs and possessed the potential to migrate from tumors to LNs. LAMP3+ DCs also expressed diverse immune-relevant ligands and exhibited potential to regulate multiple subtypes of lymphocytes. Of the macrophages in tumors that exhibited distinct transcriptional states, tumor-associated macrophages (TAMs) were associated with poor prognosis, and we established the inflammatory role of SLC40A1 and GPNMB in these cells. Further, myeloid and lymphoid cells in ascites were predominantly linked to tumor and blood origins, respectively. The dynamic properties of diverse CD45+ cell types revealed by this study add new dimensions to the immune landscape of HCC.Entities:
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Year: 2019 PMID： 31675496 DOI： 10.1016/j.cell.2019.10.003
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582