Verisa Chowjarean1,2, Tassanee Prueksasit3, Keerati Joyjamras4,5,6, Pithi Chanvorachote4,6. 1. Cosmeceutical Research, Development and Testing Center, College of Pharmacy, Rangsit University, Pathumthani, Thailand. 2. Department of Pharmaceutical Technology, College of Pharmacy, Rangsit University, Pathumthani, Thailand. 3. Department of Environmental Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand. 4. Cell-based Drug and Health Products Development Research Unit, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand pithi.c@chula.ac.th j_keerati@hotmail.com. 5. Doctor of Philosophy Program in Pharmaceutical Sciences and Technology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand. 6. Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
Abstract
Background/Aim: Fine airborne particles of Particular Matter of less than 2.5 micrometers (PM 2.5 ) have been recognized as a dominant air contamination causing critical health concerns. Herein, we determined whether isovitexin, a natural plant-derived compound could protect PM2.5-mediated oxidative stress and induce stemness in epidermal cells. Materials and Methods: Cell viability was detected by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Reactive oxygen species (ROS) were determined by flow cytometry with 2',7'-dichlorofluorescin diacetate (DCFH-DA). Protein hallmarks of stem cells were examined by western blot analysis. Results: PM2.5 treatment for 30 min increased the levels of intracellular ROS. Pre-treatment of cells with 10-50 μM of isovitexin dramatically inhibited the ROS induced by PM2.5. Antioxidant efficacy of isovitexin was also determined by the ROS scavenging activity against 2,2-diphenyl-2-picrylhydrazyl (DPPH), ABTS and superoxide anion radicals. In addition, we found that isovitexin enhanced the stem cell properties of keratinocytes, indicated by the significant increase in the levels of stem cell proteins. Conclusion: Isovitexin can be potentially used as an effective compound for preventing skin damage. Copyright
Background/Aim: Fine airborne particles of Particular Matter of less than 2.5 micrometers (PM 2.5 ) have been recognized as a dominant air contamination causing critical health concerns. Herein, we determined whether isovitexin, a natural plant-derived compound could protect PM2.5-mediated oxidative stress and induce stemness in epidermal cells. Materials and Methods: Cell viability was detected by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Reactive oxygen species (ROS) were determined by flow cytometry with 2',7'-dichlorofluorescin diacetate (DCFH-DA). Protein hallmarks of stem cells were examined by western blot analysis. Results: PM2.5 treatment for 30 min increased the levels of intracellular ROS. Pre-treatment of cells with 10-50 μM of isovitexin dramatically inhibited the ROS induced by PM2.5. Antioxidant efficacy of isovitexin was also determined by the ROS scavenging activity against 2,2-diphenyl-2-picrylhydrazyl (DPPH), ABTS and superoxide anion radicals. In addition, we found that isovitexin enhanced the stem cell properties of keratinocytes, indicated by the significant increase in the levels of stem cell proteins. Conclusion:Isovitexin can be potentially used as an effective compound for preventing skin damage. Copyright
Authors: Irini M Dijkhoff; Barbara Drasler; Bedia Begum Karakocak; Alke Petri-Fink; Giuseppe Valacchi; Marc Eeman; Barbara Rothen-Rutishauser Journal: Part Fibre Toxicol Date: 2020-07-25 Impact factor: 9.400