C Y Choo1, N Y Sulong, F Man, T W Wong. 1. MedChem Herbal Research Group, Faculty of Pharmacy, Universiti Teknologi MARA, 42300 Puncak Alam, Selangor, Malaysia. choo715@puncakalam.uitm.edu.my
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Ficus deltoidea are used as a traditional medicine by diabetes patients in Malaysia. AIM OF THE STUDY: The objective of the study is to identify and evaluate bioactive constituents with in vivo α-glucosidase inhibition. MATERIALS AND METHODS: The partitioned extracts, subfractions and pure bioactive constituents were subjected to α-glucosidase inhibition assay. The identified bioactive constituents were administered orally to sucrose loaded normoglycemic mice and induced diabetic rats. The postprandial blood glucose levels were monitored at 30 min interval. Acute toxicity was evaluated in both normoglycemic mice and induced diabetic rats. RESULTS: Bioactivity guided fractionation led to the isolation of both vitexin (1) and isovitexin (2). Oral administration of 1mg/kg of either vitexin (1) or isovitexin (2) significantly (p<0.05) reduced the postprandial blood glucose level in sucrose loaded normoglycemic mice at 30 min. The percentage of postprandial blood glucose reduction was highest in sucrose loaded induced diabetic rats administered orally with 200mg/kg of vitexin (1) or 100mg/kg of isovitexin (2). Both vitexin (1) and isovitexin (2) did not exert any signs of toxicity at the highest dose of 2g/kg administered orally to normoglycemic mice and induced diabetic rats. CONCLUSION: Both the C-glycosyl bioflavonoids, namely, vitexin (1) and isovitexin (2) exhibited in vivo α-glucosidase inhibition.
ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Ficus deltoidea are used as a traditional medicine by diabetespatients in Malaysia. AIM OF THE STUDY: The objective of the study is to identify and evaluate bioactive constituents with in vivo α-glucosidase inhibition. MATERIALS AND METHODS: The partitioned extracts, subfractions and pure bioactive constituents were subjected to α-glucosidase inhibition assay. The identified bioactive constituents were administered orally to sucrose loaded normoglycemic mice and induced diabeticrats. The postprandial blood glucose levels were monitored at 30 min interval. Acute toxicity was evaluated in both normoglycemic mice and induced diabeticrats. RESULTS: Bioactivity guided fractionation led to the isolation of both vitexin (1) and isovitexin (2). Oral administration of 1mg/kg of either vitexin (1) or isovitexin (2) significantly (p<0.05) reduced the postprandial blood glucose level in sucrose loaded normoglycemic mice at 30 min. The percentage of postprandial blood glucose reduction was highest in sucrose loaded induced diabeticrats administered orally with 200mg/kg of vitexin (1) or 100mg/kg of isovitexin (2). Both vitexin (1) and isovitexin (2) did not exert any signs of toxicity at the highest dose of 2g/kg administered orally to normoglycemic mice and induced diabeticrats. CONCLUSION: Both the C-glycosyl bioflavonoids, namely, vitexin (1) and isovitexin (2) exhibited in vivo α-glucosidase inhibition.
Authors: Olfa Belguith-Hadriche; Sonda Ammar; Maria Del Mar Contreras; Mouna Turki; Antonio Segura-Carretero; Abdelfattah El Feki; Fatma Makni-Ayedi; Mohamed Bouaziz Journal: Plant Foods Hum Nutr Date: 2016-06 Impact factor: 3.921