Saniye Göknil Çalık1, Mustafa Çalık2, Zümrüt Ela Arslan Kaşdoğan3, Mustafa Cihat Avunduk4, Olgun Kadir Arıbaş5, Hidir Esme1. 1. DepartmentofEmergencyandFirstAid,VocationalSchoolofHealthServices,KTOKaratay University,Konya,Turkey 2. Department of Thoracic Surgery, Konya Training and Research Hospital, Health Sciences University, Konya, Turkey 3. Department of Anesthesiology and Reanimation, Health Sciences University, Konya Training and Research Hospital, Konya, Turkey 4. Department of Pathology, Meram Faculty of Medicine, Necmettin Erbakan University, Konya, Turkey 5. Department of Thoracic Surgery, Faculty of Medicine, Gazi University, Ankara, Turkey
Abstract
Background/aim: We aimed to investigate the topical application of mitomycin-C (MMC) after the conventional tracheostomy in a rabbit model. Materials and methods: Twenty-four male New Zealand White rabbits were randomly divided among 3 equal groups (n: 8). Trache- ostomies were performed on 16 subjects. Group 1 which served as a control for all tracheal measurements. After tracheostomy, we applied sterile saline (group 2) or MMC at 0.8 mg/mL (group 3) around the tracheotomy site for 5 min. At the 3rd week after surgery, all tracheas were subjected to morphometric and histopathological examinations, including tracheal lumen diameter (LD), number of capillary vessels (CV), subepithelial tissue thickness (SETT), fibroblasts, and inflammatory cells (IC). Results: There was a statistically significant difference between the two tracheostomy groups themselves and the control group for LD (p = 0.035), CV (p = 0.006), SETT, fibroblasts, and IC (p < 0.001). Histopathological analysis showed the decreased LD, CV, SETT, IC, and fibroblasts compared to MMC with tracheostomy groups. MMC was more effective than saline for LD, CV, SETT, IC, and fibroblasts. Conclusion: Wound healing modulation may prevent scar formation. Fibrosis decreased following tracheostomy in the group treated with MMC. Fibroblasts appear to be key cells mediating these effects. This work is licensed under a Creative Commons Attribution 4.0 International License.
Background/aim: We aimed to investigate the topical application of mitomycin-C (MMC) after the conventional tracheostomy in a rabbit model. Materials and methods: Twenty-four male New Zealand White rabbits were randomly divided among 3 equal groups (n: 8). Trache- ostomies were performed on 16 subjects. Group 1 which served as a control for all tracheal measurements. After tracheostomy, we applied sterile saline (group 2) or MMC at 0.8 mg/mL (group 3) around the tracheotomy site for 5 min. At the 3rd week after surgery, all tracheas were subjected to morphometric and histopathological examinations, including tracheal lumen diameter (LD), number of capillary vessels (CV), subepithelial tissue thickness (SETT), fibroblasts, and inflammatory cells (IC). Results: There was a statistically significant difference between the two tracheostomy groups themselves and the control group for LD (p = 0.035), CV (p = 0.006), SETT, fibroblasts, and IC (p < 0.001). Histopathological analysis showed the decreased LD, CV, SETT, IC, and fibroblasts compared to MMC with tracheostomy groups. MMC was more effective than saline for LD, CV, SETT, IC, and fibroblasts. Conclusion: Wound healing modulation may prevent scar formation. Fibrosis decreased following tracheostomy in the group treated with MMC. Fibroblasts appear to be key cells mediating these effects. This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors: Miloslav Marel; Zdenek Pekarek; Irena Spasova; Pavel Pafko; Jan Schutzner; Jan Betka; Ronald Pospisil Journal: Respiration Date: 2005 Nov-Dec Impact factor: 3.580