The effects of moderate exercise and overtraining on learning and memory, hippocampal inflammatory cytokine levels, and brain oxidative stress markers in rats.

To investigate the exercise intensity effects on rats' memory and learning, animals were divided into control, moderate training (MT), and overtraining (OT) groups. At training last week, learning and memory was assessed using Morris water maze (MWM) and passive avoidance (PA) tests. Finally, the rat's brains were removed for evaluating oxidative stress and inflammatory cytokines. Overtraining impaired animal's performance in MWM and PA tests. In MT group, hippocampal levels of interleukin 1 beta (IL-1β) and malondialdehyde (MDA) increased, and thiol contents in hippocampal and cortical tissues decreased compared to control. In OT group, tumor necrosis factor α, IL-1β, and C-reactive protein hippocampal levels increased, MDA and nitric oxide metabolite in hippocampal and cortical tissues increased, thiol contents, catalase and superoxide dismutase activity in hippocampal and cortical tissues decreased compared to control and MT groups. Overtraining might lead to learning and memory impairment by increasing the inflammatory cytokine and oxidative stress markers.