Farzaneh Vafaee1, Mahmoud Hosseini2, Hamid Reza Sadeghinia3, Mosa Al-Reza Hadjzadeh1, Mohammad Soukhtanloo4, Motaharah Rahimi1. 1. Neurocognitive Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Postal Code 9177948564, Iran. 2. Neurogenic Inflammation Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Postal Code 9177948564, Iran. 3. Pharmacological Research Center of Medicinal Plants and Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Postal Code 9177948564, Iran. 4. Department of Biochemistry, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Postal Code 9177948564, Iran.
Abstract
BACKGROUND: The effects of soy extract on memory as well as the oxidative damage to brain tissue induced by ischemia was investigated in ovariectomised (OVX) rats. METHODS: THE RATS WERE DIVIDED INTO: 1) Sham; 2) OVX; 3) Sham‑Ischemia; 4) OVX‑Ischemia; 5) OVX-Ischemia-S 20; and 6) OVX-Ischemia-S 60. The common carotid artery was occluded (30 minutes), and it was then re-perfused. The OVX-Ischemia-S 20 and OVX-Ischemia-S 60 groups received 20 or 60 mg/kg of soy extract for eight weeks before the ischemia. RESULTS: The Sham-Ischemia and OVX-Ischemia groups took a longer time to reach the platform while, spent a shorter time in the target quadrant (Q1) than the Sham and OVX. The escape latencies in the OVX-Ischemia-S 20 and OVX-Ischemia-S 60 groups were lower while, time spent in the Q1 was higher than that of the OVX-Ischemia. In the rotarod test, there were no significant differences between the groups. The hippocampal concentrations of malondialdehyde (MDA) in the Sham-Ischemia and OVX-Ischemia groups were higher than the Sham and OVX. Pre-treatment by 20 and 60 mg/kg of the extract reduced the MDA. CONCLUSION: It is suggested that soy prevents memory impairment and brain tissue oxidative damage due to ischemia in OVX rats.
BACKGROUND: The effects of soy extract on memory as well as the oxidative damage to brain tissue induced by ischemia was investigated in ovariectomised (OVX) rats. METHODS: THE RATS WERE DIVIDED INTO: 1) Sham; 2) OVX; 3) Sham‑Ischemia; 4) OVX‑Ischemia; 5) OVX-Ischemia-S 20; and 6) OVX-Ischemia-S 60. The common carotid artery was occluded (30 minutes), and it was then re-perfused. The OVX-Ischemia-S 20 and OVX-Ischemia-S 60 groups received 20 or 60 mg/kg of soy extract for eight weeks before the ischemia. RESULTS: The Sham-Ischemia and OVX-Ischemia groups took a longer time to reach the platform while, spent a shorter time in the target quadrant (Q1) than the Sham and OVX. The escape latencies in the OVX-Ischemia-S 20 and OVX-Ischemia-S 60 groups were lower while, time spent in the Q1 was higher than that of the OVX-Ischemia. In the rotarod test, there were no significant differences between the groups. The hippocampal concentrations of malondialdehyde (MDA) in the Sham-Ischemia and OVX-Ischemia groups were higher than the Sham and OVX. Pre-treatment by 20 and 60 mg/kg of the extract reduced the MDA. CONCLUSION: It is suggested that soy prevents memory impairment and brain tissue oxidative damage due to ischemia in OVX rats.
Authors: Sara Memarpour; Farimah Beheshti; Yousef Baghcheghi; Abbas Ali Vafaei; Mahmoud Hosseini; Ali Rashidy-Pour Journal: Neurochem Res Date: 2020-09-15 Impact factor: 3.996