Literature DB >> 31631317

Awakening dormant glycosyltransferases in CHO cells with CRISPRa.

Karen Julie la Cour Karottki1, Hooman Hefzi2,3, Kai Xiong1, Isaac Shamie2,3, Anders Holmgaard Hansen1, Songyuan Li1, Lasse Ebdrup Pedersen1, Shangzhong Li2,3,4, Jae Seong Lee5, Gyun Min Lee1,6, Helene Faustrup Kildegaard1, Nathan E Lewis2,3,4.   

Abstract

Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes. We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced. This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.
© 2019 Wiley Periodicals, Inc.

Entities:  

Keywords:  CHO; CRISPRa; Mgat3; St6gal1; glycosylation

Mesh：

Substances：

Year:  2019        PMID： 31631317      PMCID： PMC7261405          DOI： 10.1002/bit.27199

Source DB:  PubMed          Journal:  Biotechnol Bioeng        ISSN： 0006-3592            Impact factor:   4.530


  21 in total

1.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

Authors:  K J Livak; T D Schmittgen
Journal:  Methods       Date:  2001-12       Impact factor: 3.608

Review 2.  Modulating carbohydrate-protein interactions through glycoengineering of monoclonal antibodies to impact cancer physiology.

Authors:  Austin Wt Chiang; Shangzhong Li; Philipp N Spahn; Anne Richelle; Chih-Chung Kuo; Mojtaba Samoudi; Nathan E Lewis
Journal:  Curr Opin Struct Biol       Date:  2016-09-14       Impact factor: 6.809

3.  Proteomics. Tissue-based map of the human proteome.

Authors:  Mathias Uhlén; Linn Fagerberg; Björn M Hallström; Cecilia Lindskog; Per Oksvold; Adil Mardinoglu; Åsa Sivertsson; Caroline Kampf; Evelina Sjöstedt; Anna Asplund; IngMarie Olsson; Karolina Edlund; Emma Lundberg; Sanjay Navani; Cristina Al-Khalili Szigyarto; Jacob Odeberg; Dijana Djureinovic; Jenny Ottosson Takanen; Sophia Hober; Tove Alm; Per-Henrik Edqvist; Holger Berling; Hanna Tegel; Jan Mulder; Johan Rockberg; Peter Nilsson; Jochen M Schwenk; Marica Hamsten; Kalle von Feilitzen; Mattias Forsberg; Lukas Persson; Fredric Johansson; Martin Zwahlen; Gunnar von Heijne; Jens Nielsen; Fredrik Pontén
Journal:  Science       Date:  2015-01-23       Impact factor: 47.728

4.  Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference.

Authors:  Kai Xiong; Kim Fabiano Marquart; Karen Julie la Cour Karottki; Shangzhong Li; Isaac Shamie; Jae Seong Lee; Signe Gerling; Nan Cher Yeo; Alejandro Chavez; Gyun Min Lee; Nathan E Lewis; Helene Faustrup Kildegaard
Journal:  Biotechnol Bioeng       Date:  2019-04-02       Impact factor: 4.530

5.  CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes.

Authors:  Luke A Gilbert; Matthew H Larson; Leonardo Morsut; Zairan Liu; Gloria A Brar; Sandra E Torres; Noam Stern-Ginossar; Onn Brandman; Evan H Whitehead; Jennifer A Doudna; Wendell A Lim; Jonathan S Weissman; Lei S Qi
Journal:  Cell       Date:  2013-07-11       Impact factor: 41.582

6.  CRISPR-Based Targeted Epigenetic Editing Enables Gene Expression Modulation of the Silenced Beta-Galactoside Alpha-2,6-Sialyltransferase 1 in CHO Cells.

Authors:  Nicolas Marx; Clemens Grünwald-Gruber; Nina Bydlinski; Heena Dhiman; Ly Ngoc Nguyen; Gerald Klanert; Nicole Borth
Journal:  Biotechnol J       Date:  2018-06-11       Impact factor: 5.726

7.  Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool.

Authors:  Carlotta Ronda; Lasse Ebdrup Pedersen; Henning Gram Hansen; Thomas Beuchert Kallehauge; Michael J Betenbaugh; Alex Toftgaard Nielsen; Helene Faustrup Kildegaard
Journal:  Biotechnol Bioeng       Date:  2014-05-22       Impact factor: 4.530

8.  Comparison of Cas9 activators in multiple species.

Authors:  Alejandro Chavez; Marcelle Tuttle; Benjamin W Pruitt; Ben Ewen-Campen; Raj Chari; Dmitry Ter-Ovanesyan; Sabina J Haque; Ryan J Cecchi; Emma J K Kowal; Joanna Buchthal; Benjamin E Housden; Norbert Perrimon; James J Collins; George Church
Journal:  Nat Methods       Date:  2016-05-23       Impact factor: 28.547

9.  Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation.

Authors:  Max A Horlbeck; Luke A Gilbert; Jacqueline E Villalta; Britt Adamson; Ryan A Pak; Yuwen Chen; Alexander P Fields; Chong Yon Park; Jacob E Corn; Martin Kampmann; Jonathan S Weissman
Journal:  Elife       Date:  2016-09-23       Impact factor: 8.140

10.  Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.

Authors:  Luke A Gilbert; Max A Horlbeck; Britt Adamson; Jacqueline E Villalta; Yuwen Chen; Evan H Whitehead; Carla Guimaraes; Barbara Panning; Hidde L Ploegh; Michael C Bassik; Lei S Qi; Martin Kampmann; Jonathan S Weissman
Journal:  Cell       Date:  2014-10-09       Impact factor: 41.582
View more
  8 in total

Review 1.  Glycoengineering Chinese hamster ovary cells: a short history.

Authors:  Roberto Donini; Stuart M Haslam; Cleo Kontoravdi
Journal:  Biochem Soc Trans       Date:  2021-04-30       Impact factor: 5.407

2.  Restoration of DNA repair mitigates genome instability and increases productivity of Chinese hamster ovary cells.

Authors:  Philipp N Spahn; Xiaolin Zhang; Qing Hu; Huiming Lu; Nathaniel K Hamaker; Hooman Hefzi; Shangzhong Li; Chih-Chung Kuo; Yingxiang Huang; Jamie C Lee; Anthony J Davis; Peter Ly; Kelvin H Lee; Nathan E Lewis
Journal:  Biotechnol Bioeng       Date:  2022-01-05       Impact factor: 4.530

3.  Multiplex secretome engineering enhances recombinant protein production and purity.

Authors:  Stefan Kol; Daniel Ley; Tune Wulff; Marianne Decker; Johnny Arnsdorf; Sanne Schoffelen; Anders Holmgaard Hansen; Tanja Lyholm Jensen; Jahir M Gutierrez; Austin W T Chiang; Helen O Masson; Bernhard O Palsson; Bjørn G Voldborg; Lasse Ebdrup Pedersen; Helene Faustrup Kildegaard; Gyun Min Lee; Nathan E Lewis
Journal:  Nat Commun       Date:  2020-04-20       Impact factor: 14.919

4.  An optimized genome-wide, virus-free CRISPR screen for mammalian cells.

Authors:  Kai Xiong; Karen Julie la Cour Karottki; Hooman Hefzi; Songyuan Li; Lise Marie Grav; Shangzhong Li; Philipp Spahn; Jae Seong Lee; Ildze Ventina; Gyun Min Lee; Nathan E Lewis; Helene Faustrup Kildegaard; Lasse Ebdrup Pedersen
Journal:  Cell Rep Methods       Date:  2021-08-04

5.  Decoupling Growth and Protein Production in CHO Cells: A Targeted Approach.

Authors:  James S Donaldson; Matthew P Dale; Susan J Rosser
Journal:  Front Bioeng Biotechnol       Date:  2021-06-02

6.  A Chinese hamster transcription start site atlas that enables targeted editing of CHO cells.

Authors:  Isaac Shamie; Sascha H Duttke; Karen J la Cour Karottki; Claudia Z Han; Anders H Hansen; Hooman Hefzi; Kai Xiong; Shangzhong Li; Samuel J Roth; Jenhan Tao; Gyun Min Lee; Christopher K Glass; Helene Faustrup Kildegaard; Christopher Benner; Nathan E Lewis
Journal:  NAR Genom Bioinform       Date:  2021-07-13

Review 7.  Protein Glycoengineering: An Approach for Improving Protein Properties.

Authors:  Bo Ma; Xiaoyang Guan; Yaohao Li; Shiying Shang; Jing Li; Zhongping Tan
Journal:  Front Chem       Date:  2020-07-23       Impact factor: 5.221

8.  Random epigenetic modulation of CHO cells by repeated knockdown of DNA methyltransferases increases population diversity and enables sorting of cells with higher production capacities.

Authors:  Marcus Weinguny; Peter Eisenhut; Gerald Klanert; Nikolaus Virgolini; Nicolas Marx; Andreas Jonsson; Daniel Ivansson; Ann Lövgren; Nicole Borth
Journal:  Biotechnol Bioeng       Date:  2020-07-24       Impact factor: 4.395
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.