Literature DB >> 31611693

mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM.

Zhifei Fu1,2, Dingming Peng1,2, Mingshu Zhang3, Fudong Xue1, Rui Zhang4, Wenting He1, Tao Xu5,6, Pingyong Xu7,8,9.   

Abstract

Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM.

Entities:  

Mesh：

Substances：

Year:  2019        PMID： 31611693     DOI： 10.1038/s41592-019-0613-6

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  1 in total

1.  Ultramorphological and ultracytochemical studies on tubuloreticular structures in lymphoid cells.

Authors:  Z Schaff; U Heine; A J Dalton
Journal:  Cancer Res       Date:  1972-12       Impact factor: 12.701
  1 in total
  17 in total

Review 1.  One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM.

Authors:  Xavier Heiligenstein; Miriam S Lucas
Journal:  Front Cell Dev Biol       Date:  2022-06-30

2.  In-resin CLEM of Epon-embedded cells using proximity labeling.

Authors:  Takahito Sanada; Junji Yamaguchi; Yoko Furuta; Soichiro Kakuta; Isei Tanida; Yasuo Uchiyama
Journal:  Sci Rep       Date:  2022-07-01       Impact factor: 4.996

Review 3.  Transcription factor dynamics in plants: Insights and technologies for in vivo imaging.

Authors:  Yuan Zhang; Yuqing Lu; Hafez El Sayyed; Jiahui Bian; Jinxing Lin; Xiaojuan Li
Journal:  Plant Physiol       Date:  2022-05-03       Impact factor: 8.005

4.  Liquid-liquid phase separation mediates the formation of herpesvirus assembly compartments.

Authors:  Sheng Zhou; Zhifei Fu; Ziwei Zhang; Xing Jia; Guangjun Xu; Long Sun; Fei Sun; Pu Gao; Pingyong Xu; Hongyu Deng
Journal:  J Cell Biol       Date:  2022-10-17       Impact factor: 8.077

Review 5.  Plant multiscale networks: charting plant connectivity by multi-level analysis and imaging techniques.

Authors:  Xi Zhang; Yi Man; Xiaohong Zhuang; Jinbo Shen; Yi Zhang; Yaning Cui; Meng Yu; Jingjing Xing; Guangchao Wang; Na Lian; Zijian Hu; Lingyu Ma; Weiwei Shen; Shunyao Yang; Huimin Xu; Jiahui Bian; Yanping Jing; Xiaojuan Li; Ruili Li; Tonglin Mao; Yuling Jiao; Haiyun Ren; Jinxing Lin
Journal:  Sci China Life Sci       Date:  2021-03-12       Impact factor: 6.038

Review 6.  Cryogenic superresolution correlative light and electron microscopy on the frontier of subcellular imaging.

Authors:  Buyun Tian; Xiaojun Xu; Yanhong Xue; Wei Ji; Tao Xu
Journal:  Biophys Rev       Date:  2021-11-26

7.  Visualization of cytoplasmic organelles via in-resin CLEM using an osmium-resistant far-red protein.

Authors:  Isei Tanida; Soichiro Kakuta; Juan Alejandro Oliva Trejo; Yasuo Uchiyama
Journal:  Sci Rep       Date:  2020-07-09       Impact factor: 4.379

8.  Two-color in-resin CLEM of Epon-embedded cells using osmium resistant green and red fluorescent proteins.

Authors:  Isei Tanida; Yoko Furuta; Junji Yamaguchi; Soichiro Kakuta; Juan Alejandro Oliva Trejo; Yasuo Uchiyama
Journal:  Sci Rep       Date:  2020-12-14       Impact factor: 4.379

Review 9.  Single-Molecule Imaging in Living Plant Cells: A Methodological Review.

Authors:  Ai-Yu Guo; Ya-Mei Zhang; Liu Wang; Di Bai; Ya-Peng Xu; Wen-Qiang Wu
Journal:  Int J Mol Sci       Date:  2021-05-11       Impact factor: 5.923

10.  Multi-scale light microscopy/electron microscopy neuronal imaging from brain to synapse with a tissue clearing method, ScaleSF.

Authors:  Takahiro Furuta; Kenta Yamauchi; Shinichiro Okamoto; Megumu Takahashi; Soichiro Kakuta; Yoko Ishida; Aya Takenaka; Atsushi Yoshida; Yasuo Uchiyama; Masato Koike; Kaoru Isa; Tadashi Isa; Hiroyuki Hioki
Journal:  iScience       Date:  2021-12-27
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.