| Literature DB >> 31602604 |
Jessica M Walter1, Max G Schubert2, Stephanie H Kung2, Kristy Hawkins2, Darren M Platt2, Aaron D Hernday2, Tina Mahatdejkul-Meadows2, Wayne Szeto2, Sunil S Chandran2, Jack D Newman2, Andrew A Horwitz2.
Abstract
CRISPR-Cas has proven to be a powerful tool for precision genetic engineering in a variety of difficult genetic systems. In the highly tractable yeast S. cerevisiae, CRISPR-Cas can be used to conduct multiple engineering steps in parallel, allowing for engineering of complex metabolic pathways at multiple genomic loci in as little as 1 week. In addition, CRISPR-Cas can be used to consolidate multiple causal alleles into a single strain, bypassing the laborious traditional methods using marked constructs, or mating. These tools compress the engineering timeline sixfold or more, greatly increasing the productivity of the strain engineer.Entities:
Keywords: Allele; CRISPR; Cas9; Metabolic engineering; Multiplex; Synthetic biology; Yeast; gRNA
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Year: 2019 PMID： 31602604 DOI： 10.1007/978-1-4939-9736-7_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745