| Literature DB >> 315973 |
A Corrigan, R O'Kennedy, H Smyth.
Abstract
Use of the nylon wool column method to separate lymphocytes results in preparations which, while fully viable, have grossly depleted cellular levels of phospholipid, cholesterol and sialic acid. These membrane effects, which relate mainly to T cells, indicate that the method is unsuitable for use in biochemical analysis of lymphocytes.Entities:
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Year: 1979 PMID： 315973 DOI： 10.1016/0022-1759(79)90298-9
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303