| Literature DB >> 31588166 |
Joseph R Lakowicz1, Józef Kuśba2, Yibing Shen1, Joanna Malicka1, Sabato D'Auria1, Zygmunt Gryczynski1, Ignacy Gryczynski1.
Abstract
We examined the effects of metallic silver island films on resonance energy transfer (RET) between a donor and acceptor bound to double helical DNA. The donor was 4',6-diamidino-2-phenylindole (DAPI) and the acceptor was propidium iodide (PI). Proximity of the labeled DNA to the silver particles resulted in a dramatic increase in RET as seen from the emission spectra and the donor decay times. Proximity to silver particles results in an increase of the Förster distance from 35 Å to an apparent value of 166 Å. These results suggest a new type of DNA hybridization assays based on RET over distances much longer than the free-space Forster distance.Entities:
Keywords: Radiative decay engineering; fluorescence resonance energy transfer; labeled DNA; silver nanoparticles
Year: 2003 PMID： 31588166 PMCID： PMC6777950 DOI： 10.1023/A:1022306630924
Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217