Literature DB >> 31586348

Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model.

Xiaonan Zhao1, Huiyan Lu2, Pradeep K Dagur3, Karen Usdin4.   

Abstract

Analysis of individual gametes has a number of applications in the study of the mechanism of repeat expansion in mouse models of the fragile X-related disorders, as well as in mouse models of other repeat expansion diseases. This chapter describes the techniques required to isolate oocytes and male gametes of different stages of maturity, along with the techniques required to accurately determine the repeat number in these gametes.

Entities:  

Keywords:  Fluorescence-activated cell sorting; Germ cell; Oocyte; Single cell analysis; Small pool PCR; Sperm

Mesh：

Substances：

Year:  2020        PMID： 31586348      PMCID： PMC8862712          DOI： 10.1007/978-1-4939-9784-8_11

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

1.  Humane and practical implications of using carbon dioxide mixed with oxygen for anesthesia or euthanasia of rats.

Authors:  P J Danneman; S Stein; S O Walshaw
Journal:  Lab Anim Sci       Date:  1997-08

2.  A Set of Assays for the Comprehensive Analysis of FMR1 Alleles in the Fragile X-Related Disorders.

Authors:  Bruce E Hayward; Yifan Zhou; Daman Kumari; Karen Usdin
Journal:  J Mol Diagn       Date:  2016-08-12       Impact factor: 5.568

Review 3.  Fragile X spectrum disorders.

Authors:  Reymundo Lozano; Carolina Alba Rosero; Randi J Hagerman
Journal:  Intractable Rare Dis Res       Date:  2014-11

4.  Isolation and cultivation of stem cells from adult mouse testes.

Authors:  Kaomei Guan; Frieder Wolf; Alexander Becker; Wolfgang Engel; Karim Nayernia; Gerd Hasenfuss
Journal:  Nat Protoc       Date:  2009       Impact factor: 13.491

5.  Regional FMRP deficits and large repeat expansions into the full mutation range in a new Fragile X premutation mouse model.

Authors:  Ali Entezam; Rea Biacsi; Bonnie Orrison; Tapas Saha; Gloria E Hoffman; Ed Grabczyk; Robert L Nussbaum; Karen Usdin
Journal:  Gene       Date:  2007-03-16       Impact factor: 3.688

6.  The mismatch repair protein MSH2 is rate limiting for repeat expansion in a fragile X premutation mouse model.

Authors:  Rachel Adihe Lokanga; Xiao-Nan Zhao; Karen Usdin
Journal:  Hum Mutat       Date:  2014-01       Impact factor: 4.878

7.  FAN1 protects against repeat expansions in a Fragile X mouse model.

Authors:  Xiao-Nan Zhao; Karen Usdin
Journal:  DNA Repair (Amst)       Date:  2018-07-05

8.  MutLγ promotes repeat expansion in a Fragile X mouse model while EXO1 is protective.

Authors:  Xiaonan Zhao; Yongwei Zhang; Kenneth Wilkins; Winfried Edelmann; Karen Usdin
Journal:  PLoS Genet       Date:  2018-10-12       Impact factor: 5.917

9.  A MutSβ-Dependent Contribution of MutSα to Repeat Expansions in Fragile X Premutation Mice?

Authors:  Xiao-Nan Zhao; Rachel Lokanga; Kimaada Allette; Inbal Gazy; Di Wu; Karen Usdin
Journal:  PLoS Genet       Date:  2016-07-18       Impact factor: 5.917

10.  Repeat Instability in the Fragile X-Related Disorders: Lessons from a Mouse Model.

Authors:  Xiaonan Zhao; Inbal Gazy; Bruce Hayward; Elizabeth Pintado; Ye Hyun Hwang; Flora Tassone; Karen Usdin
Journal:  Brain Sci       Date:  2019-03-01
View more
  3 in total

1.  FAN1's protection against CGG repeat expansion requires its nuclease activity and is FANCD2-independent.

Authors:  Xiaonan Zhao; Huiyan Lu; Karen Usdin
Journal:  Nucleic Acids Res       Date:  2021-11-18       Impact factor: 16.971

2.  All three mammalian MutL complexes are required for repeat expansion in a mouse cell model of the Fragile X-related disorders.

Authors:  Carson J Miller; Geum-Yi Kim; Xiaonan Zhao; Karen Usdin
Journal:  PLoS Genet       Date:  2020-06-26       Impact factor: 5.917

3.  CGG Repeat Expansion, and Elevated Fmr1 Transcription and Mitochondrial Copy Number in a New Fragile X PM Mouse Embryonic Stem Cell Model.

Authors:  Inbal Gazy; Carson J Miller; Geum-Yi Kim; Karen Usdin
Journal:  Front Cell Dev Biol       Date:  2020-06-30
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.