Intraoperative contrast-enhanced ultrasound for cerebral glioma resection and the relationship between microvascular perfusion and microvessel density.

We analyzed the relationship between quantitative CEUS parameters and microvessel density (MVD) of different pathologic grades of cerebral gliomas. ICEUS was performed in 49 patients with cerebral gliomas. The enhancement characteristics of cerebral gliomas were observed before and after tumor resection. The number of microvessels was counted by immunostaining with anti-CD34. Differences in these quantitative parameters in cerebral gliomas were compared and subjected to a correlation analysis with MVD. The assessment of iCEUS parameters and tumor MVD showed that cerebral gliomas of different pathological grades had different characteristics. The time-to-peak (Tmax) was significantly shorter, the peak intensity (PI) and MVD were significantly higher in high-grade cerebral gliomas than in low-grade cerebral gliomas (p < 0.05). According to the immunostaining, PI was positively (r = 0.637) correlated with MVD and Tmax was negatively (r = -0.845) correlated with MVD. ICEUS could provid dynamic and continuous real-time imaging and quantitative data analysis of different pathological grades of cerebral gliomas, the quantitiative CEUS parameters were closely related to the MVD, and be helpful in understanding the cerebral gliomas grade and refining surgical strategy.