Jing Fu1, Wenxing Zhao2, Dongmei Guo1, Zheng Li3. 1. Department of Gastroenterology, Heze Municipal Hospital, No. 2888, West Caozhou Road, Mudan District, Heze City, 274000, Shandong Province, People's Republic of China. 2. Department of Gastroenterology, Heze Medical College, Heze City, 274000, Shandong Province, People's Republic of China. 3. Department of Gastroenterology, Heze Municipal Hospital, No. 2888, West Caozhou Road, Mudan District, Heze City, 274000, Shandong Province, People's Republic of China. hzoxdl6@163.com.
Abstract
BACKGROUND: A recent study reported a novel long non-coding RNA (lncRNA) E2F-mediated cell proliferation enhancing lncRNA (EPEL, human chromosome 4, intergenic region) plays an oncogenic role in lung cancer. AIMS: We aimed to investigate the role of lncRNA EPEL in gastric cancer. METHODS: Gene expression was analyzed by RT-qPCR and western blot. Survival analysis was performed by comparing survival curves. Cell proliferation, migration, and invasion were analyzed by CCK-8 and Transwell assays. RESULTS: We found that lncRNA EPEL and Runt-related transcription factor 2 (RUNX2) were both upregulated in gastric cancer. EPEL and RUNX2 were positively correlated in tumor. Patients with high expression level of lncRNA EPEL showed poor survival. LncRNA EPEL and RUNX2 overexpression promoted, while lncRNA EPEL siRNA silencing inhibited the migration, proliferation, and invasion of gastric cancers. In addition, RUNX2 overexpression completely rescued the inhibited cancer cell migration, proliferation, and invasion caused by lncRNA EPEL siRNA silencing. Consistently, EPEL overexpression resulted in upregulated RUNX2 expression, while RUNX2 overexpression did not affect lncRNA EPEL expression. CONCLUSIONS: Therefore, lncRNA EPEL may regulate cancer cell behaviors and affect prognosis of gastric cancer by interacting with RUNX2.
BACKGROUND: A recent study reported a novel long non-coding RNA (lncRNA) E2F-mediated cell proliferation enhancing lncRNA (EPEL, human chromosome 4, intergenic region) plays an oncogenic role in lung cancer. AIMS: We aimed to investigate the role of lncRNA EPEL in gastric cancer. METHODS: Gene expression was analyzed by RT-qPCR and western blot. Survival analysis was performed by comparing survival curves. Cell proliferation, migration, and invasion were analyzed by CCK-8 and Transwell assays. RESULTS: We found that lncRNA EPEL and Runt-related transcription factor 2 (RUNX2) were both upregulated in gastric cancer. EPEL and RUNX2 were positively correlated in tumor. Patients with high expression level of lncRNA EPEL showed poor survival. LncRNA EPEL and RUNX2 overexpression promoted, while lncRNA EPEL siRNA silencing inhibited the migration, proliferation, and invasion of gastric cancers. In addition, RUNX2 overexpression completely rescued the inhibited cancer cell migration, proliferation, and invasion caused by lncRNA EPEL siRNA silencing. Consistently, EPEL overexpression resulted in upregulated RUNX2 expression, while RUNX2 overexpression did not affect lncRNA EPEL expression. CONCLUSIONS: Therefore, lncRNA EPEL may regulate cancer cell behaviors and affect prognosis of gastric cancer by interacting with RUNX2.
Authors: Zhensheng Wang; David Y Graham; Anam Khan; Maya Balakrishnan; Hannah R Abrams; Hashem B El-Serag; Aaron P Thrift Journal: Int J Epidemiol Date: 2018-06-01 Impact factor: 7.196
Authors: Rajeev K Boregowda; Oyenike O Olabisi; Walid Abushahba; Byeong-Seon Jeong; Keneshia K Haenssen; Wenjin Chen; Marina Chekmareva; Ahmed Lasfar; David J Foran; James S Goydos; Karine A Cohen-Solal Journal: Cancer Lett Date: 2014-03-18 Impact factor: 8.679
Authors: Jitesh Pratap; Karen M Imbalzano; Jean M Underwood; Nathalie Cohet; Karthiga Gokul; Jacqueline Akech; Andre J van Wijnen; Janet L Stein; Anthony N Imbalzano; Jeffrey A Nickerson; Jane B Lian; Gary S Stein Journal: Cancer Res Date: 2009-08-18 Impact factor: 12.701
Authors: Felipe J F Coimbra; Wilson Luiz da Costa; Héber S C Ribeiro; Alessandro L Diniz; André Luís de Godoy; Igor Correia de Farias; Antonio Moris Cury Filho; Marcello Ferretti Fanelli; Maria Dirlei F S Begnami; Fernando Augusto Soares Journal: Ann Surg Oncol Date: 2015-11-05 Impact factor: 5.344