Jared T Ahrendsen1, Mariella G Filbin2, Susan N Chi2, Peter E Manley2, Karen D Wright2, Pratiti Bandopadhayay2, Jessica R Clymer2, Kee Kiat Yeo2, Mark W Kieran2,3, Robert Jones2, Hart G Lidov4, Keith L Ligon5,4, Sanda Alexandrescu6,7. 1. Department of Pathology, Beth Israel Deaconess Medical Center, Boston, USA. 2. Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, USA. 3. Brystol Meyers Squibb, New York, USA. 4. Department of Pathology, Boston Children's Hospital, Harvard Medical School, 300 Longwood Ave, Boston, MA, 02215, USA. 5. Department of Pathology, Brigham and Women's Hospital, Boston, USA. 6. Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, USA. Sanda.alexandrescu@childrens.harvard.edu. 7. Department of Pathology, Boston Children's Hospital, Harvard Medical School, 300 Longwood Ave, Boston, MA, 02215, USA. Sanda.alexandrescu@childrens.harvard.edu.
Abstract
BACKGROUND: Pediatric brain tumors are associated with high morbidity and mortality, in part due to insufficient understanding of tumor biology. With limited tissue allocation for research from surgical specimens, a key barrier to improving biological understanding, brain tumor autopsies have become an increasingly valuable resource. This study reviews the brain tumor autopsy practice at our institution and describes specific emerging research utilization patterns beyond the clinical autopsy report. METHODS: We performed a retrospective analysis of brain tumor autopsies at Boston Children's Hospital (BCH) between 2007 and 2017 and reviewed their consents, neuropathology reports and final diagnoses. We reviewed the method of tissue triaging for research consented autopsies (bioregistry, frozen and fresh tissue) and documented their specific uses. RESULTS: Ninety-six deaths at BCH were due to brain tumors; 56 autopsies were performed (58.3%), of which 49 (87.5%) were consented for research. Tumor mapping was performed on all cases and tissue was allocated for DNA- and RNA-based sequencing studies (published and ongoing). Three tissue allocations with a postmortem interval of 8 h or less resulted in successful cell lines. Tissue from 14 autopsies was contributed to the National DIPG Registry. CONCLUSION: Our institutional pediatric brain tumor autopsy clinical experience demonstrates the increased utility and wide utilization of autopsy-derived tissue for multiple types of research. These results support the increased efforts to obtain research consent for brain tumor autopsy and active collection of unfixed autopsy material in the molecular era.
BACKGROUND: Pediatric brain tumors are associated with high morbidity and mortality, in part due to insufficient understanding of tumor biology. With limited tissue allocation for research from surgical specimens, a key barrier to improving biological understanding, brain tumor autopsies have become an increasingly valuable resource. This study reviews the brain tumor autopsy practice at our institution and describes specific emerging research utilization patterns beyond the clinical autopsy report. METHODS: We performed a retrospective analysis of brain tumor autopsies at Boston Children's Hospital (BCH) between 2007 and 2017 and reviewed their consents, neuropathology reports and final diagnoses. We reviewed the method of tissue triaging for research consented autopsies (bioregistry, frozen and fresh tissue) and documented their specific uses. RESULTS: Ninety-six deaths at BCH were due to brain tumors; 56 autopsies were performed (58.3%), of which 49 (87.5%) were consented for research. Tumor mapping was performed on all cases and tissue was allocated for DNA- and RNA-based sequencing studies (published and ongoing). Three tissue allocations with a postmortem interval of 8 h or less resulted in successful cell lines. Tissue from 14 autopsies was contributed to the National DIPG Registry. CONCLUSION: Our institutional pediatric brain tumor autopsy clinical experience demonstrates the increased utility and wide utilization of autopsy-derived tissue for multiple types of research. These results support the increased efforts to obtain research consent for brain tumor autopsy and active collection of unfixed autopsy material in the molecular era.
Authors: Anuja Ghorpade; Leslie Bruch; Yuri Persidsky; Betty Chin; William H C Brown; Kathleen Borgmann; Raisa Persidsky; Li Wu; Spring Holter; Robin Cotter; Jill Faraci; David Heilman; VaKara Meyer; Jane F Potter; Susan Swindells; Howard E Gendelman Journal: J Neuroimmunol Date: 2005-04-07 Impact factor: 3.478
Authors: C M Hulette; K A Welsh-Bohmer; B Crain; M H Szymanski; N O Sinclaire; A D Roses Journal: Arch Pathol Lab Med Date: 1997-06 Impact factor: 5.534
Authors: Patricia K Riggs; Antoine Chaillon; Guochun Jiang; Scott L Letendre; Yuyang Tang; Jeff Taylor; Andrew Kaytes; Davey M Smith; Karine Dubé; Sara Gianella Journal: Curr HIV/AIDS Rep Date: 2022-10-19 Impact factor: 5.495