Santiago Arango-Santander1, Carolina Montoya2, Alejandro Pelaez-Vargas3, E Alexander Ossa4. 1. Faculty of Dentistry, Universidad Cooperativa de Colombia. Carrera 47 # 37 sur 18, Envigado, Colombia. Electronic address: santiago.arango@campusucc.edu.co. 2. Production Engineering Department, Universidad Eafit, Carrera 49 # 7 sur 50, Medellín, Colombia. Electronic address: cmonto18@eafit.edu.co. 3. Faculty of Dentistry, Universidad Cooperativa de Colombia. Carrera 47 # 37 sur 18, Envigado, Colombia. Electronic address: alejandro.pelaezv@campusucc.edu.co. 4. Production Engineering Department, Universidad Eafit, Carrera 49 # 7 sur 50, Medellín, Colombia. Electronic address: eossa@eafit.edu.co.
Abstract
OBJECTIVE: The purpose of this investigation was to establish microstructure, microhardness, fracture toughness, chemical composition, and crack repair of bovine enamel and to compare these features with their human counterparts. DESIGN: Bovine enamel fragments were prepared and optical microscopy and atomic force microscopy were used to establish microstructure; Raman spectroscopy was used to estimate composition and microindentation using Vickers testing was performed to evaluate hardness. RESULTS: A strong dependence between indentation load and microhardness values was observed, as was the case in human enamel. Similar microstructure and chemical composition between bovine and human enamel, 7.89% lower microhardness and 40% higher fracture toughness values for bovine enamel were found. CONCLUSION: From a structural and mechanical standpoint, bovine enamel is a suitable alternative to human enamel for in vitro testing of dental products.
OBJECTIVE: The purpose of this investigation was to establish microstructure, microhardness, fracture toughness, chemical composition, and crack repair of bovine enamel and to compare these features with their human counterparts. DESIGN:Bovine enamel fragments were prepared and optical microscopy and atomic force microscopy were used to establish microstructure; Raman spectroscopy was used to estimate composition and microindentation using Vickers testing was performed to evaluate hardness. RESULTS: A strong dependence between indentation load and microhardness values was observed, as was the case in human enamel. Similar microstructure and chemical composition between bovine and human enamel, 7.89% lower microhardness and 40% higher fracture toughness values for bovine enamel were found. CONCLUSION: From a structural and mechanical standpoint, bovine enamel is a suitable alternative to human enamel for in vitro testing of dental products.
Authors: Johannes Mischo; Thomas Faidt; Ryan B McMillan; Johanna Dudek; Gubesh Gunaratnam; Pardis Bayenat; Anne Holtsch; Christian Spengler; Frank Müller; Hendrik Hähl; Markus Bischoff; Matthias Hannig; Karin Jacobs Journal: ACS Biomater Sci Eng Date: 2022-03-09