Literature DB >> 3156375

Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes.

R L Gourse, Y Takebe, R A Sharrock, M Nomura.   

Abstract

We have constructed a conditional rRNA gene expression system by fusing a plasmid-encoded rrnB operon to the lambda PL promoter/operator. It was thereby possible to study the events that lead to the regulation of chromosomal rRNA and tRNA synthesis after overproduction of rRNA. rRNA induction resulted in a 2-fold increase in 30S and 50S free ribosomal subunits, whereas the polysome fraction was unaffected. Overproduction of rRNA and "free" ribosomes produced a large repression of rRNA and tRNA synthesis from chromosomal genes and a smaller increase in the concentration of guanosine tetraphosphate. These results lend support to the ribosome feedback regulation model: rRNA and tRNA operons are negatively regulated, either directly or through some intermediate, by free, nontranslating ribosomes.

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Year:  1985        PMID： 3156375      PMCID： PMC397195          DOI： 10.1073/pnas.82.4.1069

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

Review 1.  Regulation of bacterial growth, RNA, and protein synthesis.

Authors:  D P Nierlich
Journal:  Annu Rev Microbiol       Date:  1978       Impact factor: 15.500

2.  Expression of spacer tRNA genes in ribosomal RNA transcription units carried by hybrid Col E1 plasmids in E. coli.

Authors:  T Ikemura; M Nomura
Journal:  Cell       Date:  1977-08       Impact factor: 41.582

3.  Culture medium for enterobacteria.

Authors:  F C Neidhardt; P L Bloch; D F Smith
Journal:  J Bacteriol       Date:  1974-09       Impact factor: 3.490

4.  Evidence for reduced breakdown of messenger RNA during blocked transcription or translation in Escherichia coli.

Authors:  L Lindahl; J Forchhammer
Journal:  J Mol Biol       Date:  1969-08-14       Impact factor: 5.469

5.  Level of rRNA, not tRNA, synthesis controls transcription of rRNA and tRNA operons in Escherichia coli.

Authors:  R L Gourse; M Nomura
Journal:  J Bacteriol       Date:  1984-12       Impact factor: 3.490

6.  Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.

Authors:  E Remaut; P Stanssens; W Fiers
Journal:  Gene       Date:  1981-10       Impact factor: 3.688

7.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

8.  Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli.

Authors:  J Brosius; T J Dull; D D Sleeter; H F Noller
Journal:  J Mol Biol       Date:  1981-05-15       Impact factor: 5.469

9.  Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.

Authors:  R A Young; J A Steitz
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

10.  Purified lambda regulatory protein cII positively activates promoters for lysogenic development.

Authors:  H Simatake; M Rosenberg
Journal:  Nature       Date:  1981-07-09       Impact factor: 49.962
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  67 in total

1.  Increased rrn gene dosage causes intermittent transcription of rRNA in Escherichia coli.

Authors:  J Voulgaris; S French; R L Gourse; C Squires; C L Squires
Journal:  J Bacteriol       Date:  1999-07       Impact factor: 3.490

2.  Covariance of complementary rRNA loop nucleotides does not necessarily represent functional pseudoknot formation in vivo.

Authors:  N S Chernyaeva; E J Murgola
Journal:  J Bacteriol       Date:  2000-10       Impact factor: 3.490

3.  Heterozygous De Novo UBTF Gain-of-Function Variant Is Associated with Neurodegeneration in Childhood.

Authors:  Simon Edvardson; Claudia M Nicolae; Pankaj B Agrawal; Cyril Mignot; Katelyn Payne; Asuri Narayan Prasad; Chitra Prasad; Laurie Sadler; Caroline Nava; Thomas E Mullen; Amber Begtrup; Berivan Baskin; Zöe Powis; Avraham Shaag; Boris Keren; George-Lucian Moldovan; Orly Elpeleg
Journal:  Am J Hum Genet       Date:  2017-08-03       Impact factor: 11.025

4.  Mutational eidence for a functional connection between two domains of 23S rRNA in translation termination.

Authors:  Alexey L Arkov; Klas O F Hedenstierna; Emanuel J Murgola
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

5.  Single-base mutations at position 2661 of Escherichia coli 23S rRNA increase efficiency of translational proofreading.

Authors:  P Melançon; W E Tapprich; L Brakier-Gingras
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490

6.  How a mutation in the gene encoding sigma 70 suppresses the defective heat shock response caused by a mutation in the gene encoding sigma 32.

Authors:  Y N Zhou; C A Gross
Journal:  J Bacteriol       Date:  1992-11       Impact factor: 3.490

7.  A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.

Authors:  M O'Connor; H U Göringer; A E Dahlberg
Journal:  Nucleic Acids Res       Date:  1992-08-25       Impact factor: 16.971

8.  Isolation of antibiotic resistance mutations in the rRNA by using an in vitro selection system.

Authors:  Luisa Cochella; Rachel Green
Journal:  Proc Natl Acad Sci U S A       Date:  2004-03-04       Impact factor: 11.205

9.  The involvement of base 1054 in 16S rRNA for UGA stop codon dependent translational termination.

Authors:  A Hänfler; B Kleuvers; H U Göringer
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

Review 10.  Metabolic growth rate control in Escherichia coli may be a consequence of subsaturation of the macromolecular biosynthetic apparatus with substrates and catalytic components.

Authors:  K F Jensen; S Pedersen
Journal:  Microbiol Rev       Date:  1990-06
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