Yi Cui1, Bai Xu2, Yanyun Yin2, Bing Chen2, Yannan Zhao2, Zhifeng Xiao2, Bin Yang2, Qingyuan Meng2, Yongxiang Fang3, Qinghan Liang4, Ling Zhou5, Xu Ma1, Jianwu Dai2. 1. Reproductive & Genetic Center, National Research Institute for Family Planning, Beijing 100081, PR China. 2. Key Laboratory of Molecular Developmental Biology, Institute of Genetics & Developmental Biology, Chinese Academy of Sciences, Beijing 100080, PR China. 3. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, PR China. 4. Department of Gynaecology, Beijing haidian maternal & child health hospital, Beijing 100089, PR China. 5. Department of Gynaecology & Obstetrics, Strategic Support Force Medical Centre of PLA, Beijing 100101, PR China.
Abstract
Aim: The major symptom of many closed spinal dysraphism patients is that the laminas or arches of vertebra are not fused well. To date, the bone repair of spina bifida for young children is a significant challenge in clinical practice. Materials & methods: Bovine bone collagen particle (BBCP) scaffolds combined with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were implanted in the defect area. X-ray analysis was performed after 3 months. Tissues were harvested for gross observation, and histological and immunohistochemical staining. Results: The BBCP supported hUC-MSCs adhesion and growth. Implanted BBCP combined with hUC-MSCs also promoted bone regeneration in the vertebral lamina and arch defect area. Conclusion: This method represents a new strategy for vertebral lamina and arch reconstruction in children.
Aim: The major symptom of many closed spinal dysraphismpatients is that the laminas or arches of vertebra are not fused well. To date, the bone repair of spina bifida for young children is a significant challenge in clinical practice. Materials & methods: Bovine bone collagen particle (BBCP) scaffolds combined with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were implanted in the defect area. X-ray analysis was performed after 3 months. Tissues were harvested for gross observation, and histological and immunohistochemical staining. Results: The BBCP supported hUC-MSCs adhesion and growth. Implanted BBCP combined with hUC-MSCs also promoted bone regeneration in the vertebral lamina and arch defect area. Conclusion: This method represents a new strategy for vertebral lamina and arch reconstruction in children.
Entities:
Keywords:
BBCP; bone regeneration; hUC-MSCs; lamina vertebral defects; weaned rabbit
Authors: Ahmad Jabir Rahyussalim; Ahmad Nugroho; Muhammad Luqman Labib Zufar; Irfan Fathurrahman; Tri Kurniawati Journal: Stem Cells Int Date: 2021-08-23 Impact factor: 5.443
Authors: Sarah C Stokes; Jordan E Jackson; Christina M Theodorou; Christopher D Pivetti; Priyadarsini Kumar; Kaeli J Yamashiro; Aijun Wang; Diana L Farmer Journal: Fetal Diagn Ther Date: 2021-06-10 Impact factor: 2.208