The zymogram technique: isoenzyme patterns as an aid in Penicillium classification.

The zymogram technique, a means of visualizing isoenzymes and comparing differences between related species, is described. The technique has been applied to the very difficult Penicillium subgenus Penicillium with notable success. Results have reinforced some aspects of accepted taxonomies and provided a basis for re-examination of others. The zymogram technique should have broad applicability to other fungal genera.