Literature DB >> 31482872

Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling.

Dmitry Torchinsky1, Yael Michaeli, Natalie R Gassman, Yuval Ebenstein.   

Abstract

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight.

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Year:  2019        PMID： 31482872      PMCID： PMC6783632          DOI： 10.1039/c9cc05198h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  42 in total

1.  Lighting up individual DNA damage sites by in vitro repair synthesis.

Authors:  Shahar Zirkin; Sivan Fishman; Hila Sharim; Yael Michaeli; Jeremy Don; Yuval Ebenstein
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Review 2.  Immunological methods for detection of carcinogen-DNA damage in humans.

Authors:  R M Santella
Journal:  Cancer Epidemiol Biomarkers Prev       Date:  1999-09       Impact factor: 4.254

3.  Induction of oxidative DNA base damage in human skin cells by UV and near visible radiation.

Authors:  E Kvam; R M Tyrrell
Journal:  Carcinogenesis       Date:  1997-12       Impact factor: 4.944

Review 4.  Cancer risk and oxidative DNA damage in man.

Authors:  S Loft; H E Poulsen
Journal:  J Mol Med (Berl)       Date:  1996-06       Impact factor: 4.599

5.  Oxidative damage to mitochondrial DNA is increased in Alzheimer's disease.

Authors:  P Mecocci; U MacGarvey; M F Beal
Journal:  Ann Neurol       Date:  1994-11       Impact factor: 10.422

6.  Analysis of alcohol-induced DNA damage in Escherichia coli by visualizing single genomic DNA molecules.

Authors:  Yujin Kang; Jinyong Lee; Jisoo Kim; Yeeun Oh; Dogeun Kim; Jungyun Lee; Sangyong Lim; Kyubong Jo
Journal:  Analyst       Date:  2016-05-17       Impact factor: 4.616

7.  How different wavelengths of the ultraviolet spectrum contribute to skin carcinogenesis: the role of cellular damage responses.

Authors:  Thomas M Rünger
Journal:  J Invest Dermatol       Date:  2007-09       Impact factor: 8.551

Review 8.  Endogenous DNA damage in humans: a review of quantitative data.

Authors:  Rinne De Bont; Nik van Larebeke
Journal:  Mutagenesis       Date:  2004-05       Impact factor: 3.000

Review 9.  UV radiation and the skin.

Authors:  John D'Orazio; Stuart Jarrett; Alexandra Amaro-Ortiz; Timothy Scott
Journal:  Int J Mol Sci       Date:  2013-06-07       Impact factor: 5.923

10.  Long-read single-molecule maps of the functional methylome.

Authors:  Hila Sharim; Assaf Grunwald; Tslil Gabrieli; Yael Michaeli; Sapir Margalit; Dmitry Torchinsky; Rani Arielly; Gil Nifker; Matyas Juhasz; Felix Gularek; Miguel Almalvez; Brandon Dufault; Sreetama Sen Chandra; Alexander Liu; Surajit Bhattacharya; Yi-Wen Chen; Eric Vilain; Kathryn R Wagner; Jonathan Pevsner; Jeff Reifenberger; Ernest T Lam; Alex R Hastie; Han Cao; Hayk Barseghyan; Elmar Weinhold; Yuval Ebenstein
Journal:  Genome Res       Date:  2019-03-07       Impact factor: 9.043
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  10 in total

1.  DNA damage measurements within tissue samples with Repair Assisted Damage Detection (RADD).

Authors:  Kevin J Lee; Elise Mann; Luciana Madeira da Silva; Jennifer Scalici; Natalie R Gassman
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Review 2.  Methodologies for detecting environmentally induced DNA damage and repair.

Authors:  Wentao Li; Aziz Sancar
Journal:  Environ Mol Mutagen       Date:  2020-02-29       Impact factor: 3.216

3.  Single-molecule optical genome mapping in nanochannels: multidisciplinarity at the nanoscale.

Authors:  Jonathan Jeffet; Sapir Margalit; Yael Michaeli; Yuval Ebenstein
Journal:  Essays Biochem       Date:  2021-04-16       Impact factor: 8.000

4.  Associations between DNA Damage and PD-L1 Expression in Ovarian Cancer, a Potential Biomarker for Clinical Response.

Authors:  Elise K Mann; Kevin J Lee; Dongquan Chen; Luciana Madeira da Silva; Valeria L Dal Zotto; Jennifer Scalici; Natalie R Gassman
Journal:  Biology (Basel)       Date:  2021-04-29

5.  From single-molecule to genome-wide mapping of DNA lesions: repair-assisted damage detection sequencing.

Authors:  Noa Gilat; Dena Fridman; Hila Sharim; Sapir Margalit; Natalie R Gassman; Yael Michaeli; Yuval Ebenstein
Journal:  Biophys Rep       Date:  2021-12-08

6.  Repair-Assisted Damage Detection Reveals Biological Disparities in Prostate Cancer between African Americans and European Americans.

Authors:  Kimiko L Krieger; Jie H Gohlke; Kevin J Lee; Danthasinghe Waduge Badrajee Piyarathna; Patricia D Castro; Jeffrey A Jones; Michael M Ittmann; Natalie R Gassman; Arun Sreekumar
Journal:  Cancers (Basel)       Date:  2022-02-17       Impact factor: 6.639

7.  Chemoenzymatic labeling of DNA methylation patterns for single-molecule epigenetic mapping.

Authors:  Tslil Gabrieli; Yael Michaeli; Sigal Avraham; Dmitry Torchinsky; Sapir Margalit; Leonie Schütz; Matyas Juhasz; Ceyda Coruh; Nissim Arbib; Zhaohui Sunny Zhou; Julie A Law; Elmar Weinhold; Yuval Ebenstein
Journal:  Nucleic Acids Res       Date:  2022-09-09       Impact factor: 19.160

Review 8.  Miniaturized Chemical Tags for Optical Imaging.

Authors:  Sam Benson; Fabio de Moliner; William Tipping; Marc Vendrell
Journal:  Angew Chem Int Ed Engl       Date:  2022-07-21       Impact factor: 16.823

9.  Quantifying DNA damage induced by ionizing radiation and hyperthermia using single DNA molecule imaging.

Authors:  Vandana Singh; Pegah Johansson; Dmitry Torchinsky; Yii-Lih Lin; Robin Öz; Yuval Ebenstein; Ola Hammarsten; Fredrik Westerlund
Journal:  Transl Oncol       Date:  2020-07-08       Impact factor: 4.243

10.  Click and Cut: a click chemistry approach to developing oxidative DNA damaging agents.

Authors:  Natasha McStay; Creina Slator; Vandana Singh; Alex Gibney; Fredrik Westerlund; Andrew Kellett
Journal:  Nucleic Acids Res       Date:  2021-10-11       Impact factor: 16.971
  10 in total

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