Characterization of the complete mitochondrial genomes of two species of the genus Aphaena Guérin-Méneville (Hemiptera: Fulgoridae) and its phylogenetic implications.

The complete mitochondrial genomes (mitogenomes) of Aphaena (Callidepsa) amabilis and Aphaena (Aphaena) discolor nigrotibiata were sequenced. The mitogenomes of these two species are 16,237 bp and 16,116 bp in length with an A + T content of 77.9% and 77.0%, respectively. Each contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs) and a control region (A + T-rich region). All PCGs initiate with the standard start codon of ATN and terminate with the complete stop codon of TAA or TAG except for atp6, where nad1 ends with an incomplete T codon. All tRNAs have the typical clover-leaf structure except for trnS1 and trnV which have a reduced DHU arm. Moreover, these two mitogenomes have trnL2, trnR and trnT with an unpaired base in the acceptor stem. The putative A + T-rich region includes multiple types of tandem repeat regions. These phylogenetic analyses are reconstructed based on 13 protein-coding genes of 25 auchenorrhynchan mitogenomes, with both maximum likelihood and Bayesian analyses yielding robust identical phylogenetic trees. These results support a monophyletic Auchenorrhyncha and the relationship (Pyrops + (Lycorma + Aphaena)) within Fulgoridae.