Dorsaf Moalla Rekik1, Sameh Ben Khedir2, Amal Daoud3, Kamilia Ksouda Moalla4, Tarak Rebai2, Zouheir Sahnoun4. 1. Laboratory of Pharmacology, Faculty of Medicine, University of Sfax, Sfax, Tunisia, rmdorsaf@gmail.com. 2. Laboratory of Histology-Embryology, Faculty of Medicine, University of Sfax, Sfax, Tunisia. 3. Laboratory of Plant Biotechnology, Faculty of Science, University of Sfax, Sfax, Tunisia. 4. Laboratory of Pharmacology, Faculty of Medicine, University of Sfax, Sfax, Tunisia.
Abstract
BACKGROUND: Lawsonia inermis-extracted oil is known for therapeutic properties, especially wound healing. This study assesses the potential of this oil for wound healing in a rat model. METHODOLOGY: To assess the potential of L. inermis-extracted oil for wound healing, phytochemical, antibacterial, and antioxidant analyses were conducted. Uniform wound excision was induced on the dorsum of randomly selected rats divided into 3 groups cleaned and treated with saline solution (control), Cicaflora (reference), and L. inermisoil. Biopsies performed after healing were histologically assessed. CONCLUSIONS: The quality and content of the fatty acids in the oil were determined. Results showed a high content of bioactive components inducing an efficient wound healing effect determined by an in vivo study. Histological and chromatic assessment findings revealed healing in the oil-treated group but not in the untreated group, a full reepithelialization with reappearance of skin appendages and well-organized collagen fibers without any inflammatory cells. This might be due to a synergistic effect of the phytoconstituents present in the oil.
BACKGROUND:Lawsonia inermis-extracted oil is known for therapeutic properties, especially wound healing. This study assesses the potential of this oil for wound healing in a rat model. METHODOLOGY: To assess the potential of L. inermis-extracted oil for wound healing, phytochemical, antibacterial, and antioxidant analyses were conducted. Uniform wound excision was induced on the dorsum of randomly selected rats divided into 3 groups cleaned and treated with saline solution (control), Cicaflora (reference), and L. inermisoil. Biopsies performed after healing were histologically assessed. CONCLUSIONS: The quality and content of the fatty acids in the oil were determined. Results showed a high content of bioactive components inducing an efficient wound healing effect determined by an in vivo study. Histological and chromatic assessment findings revealed healing in the oil-treated group but not in the untreated group, a full reepithelialization with reappearance of skin appendages and well-organized collagen fibers without any inflammatory cells. This might be due to a synergistic effect of the phytoconstituents present in the oil.
Authors: Dalia Elebeedy; Aml Ghanem; Marwa El-Sayed; Eman Fayad; Ola A Abu Ali; Amal Alyamani; Asmaa Sayed Abdelgeliel Journal: Infect Drug Resist Date: 2022-02-19 Impact factor: 4.003