| Literature DB >> 31455787 |
Heike Lange1, Simon Y A Ndecky2, Carlos Gomez-Diaz2, David Pflieger2, Nicolas Butel3, Julie Zumsteg2, Lauriane Kuhn4, Christina Piermaria4, Johana Chicher4, Michael Christie5, Ezgi S Karaaslan5, Patricia L M Lang5, Detlef Weigel5, Hervé Vaucheret3, Philippe Hammann4, Dominique Gagliardi6.
Abstract
The RNA exosome is a key 3'-5' exoribonuclease with an evolutionarily conserved structure and function. Its cytosolic functions require the co-factors SKI7 and the Ski complex. Here we demonstrate by co-purification experiments that the ARM-repeat protein RESURRECTION1 (RST1) and RST1 INTERACTING PROTEIN (RIPR) connect the cytosolic Arabidopsis RNA exosome to the Ski complex. rst1 and ripr mutants accumulate RNA quality control siRNAs (rqc-siRNAs) produced by the post-transcriptional gene silencing (PTGS) machinery when mRNA degradation is compromised. The small RNA populations observed in rst1 and ripr mutants are also detected in mutants lacking the RRP45B/CER7 core exosome subunit. Thus, molecular and genetic evidence supports a physical and functional link between RST1, RIPR and the RNA exosome. Our data reveal the existence of additional cytosolic exosome co-factors besides the known Ski subunits. RST1 is not restricted to plants, as homologues with a similar domain architecture but unknown function exist in animals, including humans.Entities:
Mesh：
Substances：
Year: 2019 PMID： 31455787 PMCID： PMC6711988 DOI： 10.1038/s41467-019-11807-4
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919