| Literature DB >> 31451765 |
Shiqian Li1,2, Xavier Prasanna3, Veijo T Salo1,2, Ilpo Vattulainen3,4, Elina Ikonen5,6.
Abstract
Auxin-inducible degron technology allows rapid and controlled protein depletion. However, basal degradation without auxin and inefficient auxin-inducible depletion have limited its utility. We have identified a potent auxin-inducible degron system composed of auxin receptor F-box protein AtAFB2 and short degron miniIAA7. The system showed minimal basal degradation and enabled rapid auxin-inducible depletion of endogenous human transmembrane, cytoplasmic and nuclear proteins in 1 h with robust functional phenotypes.Entities:
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Year: 2019 PMID： 31451765 DOI： 10.1038/s41592-019-0512-x
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547