| Literature DB >> 31444744 |
Ananya Paul1, Caterina Musetti1,2, Rupesh Nanjunda1,3, W David Wilson4.
Abstract
Biosensor-surface plasmon resonance (SPR) technology is now well established as a quantitative approach for the study of nucleic acid interactions in real time, without the need for labeling any components of the interaction. The method provides real-time equilibrium and kinetic characterization for quadruplex DNA interactions and requires small amounts of materials and no external probe. A detailed protocol for quadruplex-DNA interaction analyses with a variety of binding molecules using biosensor-SPR methods is presented. Explanations of the SPR method with basic fundamentals for use and analysis of results are described with recommendations on the preparation of the SPR instrument, sensor chips, and samples. Details of experimental design, quantitative and qualitative data analyses, and presentation are described. Some specific examples of small molecule-DNA quadruplex interactions are presented with results evaluated by both kinetic and steady-state SPR methods.Entities:
Keywords: Biosensor; Kinetics; Mass transfer; Small molecule-nucleic acid interactions; Steady-state analysis; Surface plasmon resonance
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Year: 2019 PMID： 31444744 PMCID： PMC6774244 DOI： 10.1007/978-1-4939-9666-7_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745