Huai Huang1, Li-Ying Shi2, Li-Liang Wei3, Yu-Shuai Han4, Wen-Jing Yi5, Zhi-Wen Pan6, Ting-Ting Jiang7, Jing Chen8, Hui-Hui Tu9, Zhi-Bin Li10, Yu-Ting Hu11, Ji-Cheng Li12. 1. School of Medicine, South China University of Technology, Guangzhou 510006, PR China; Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: yichengshuian5653@163.com. 2. Department of Clinical Laboratory, Zhejiang Hospital, Hangzhou 310013, PR China. Electronic address: shi-lee-ying@163.com. 3. Department of Pneumology, Shaoxing Municipal Hospital, Shaoxing 312000, PR China. Electronic address: weililiang1981@163.com. 4. Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: hyshuaizd@zju.edu.cn. 5. Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: 18268178640@163.com. 6. Department of Clinical Laboratory, Zhejiang Cancer Hospital, Hangzhou 310022, PR China. Electronic address: pan-zhiwen@163.com. 7. School of Medicine, South China University of Technology, Guangzhou 510006, PR China. Electronic address: jiang_tingting1989@126.com. 8. Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: chenjing2017@zju.edu.cn. 9. Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: baiweituhuihui@163.com. 10. Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: lizhibinaa@163.com. 11. School of Medicine, South China University of Technology, Guangzhou 510006, PR China; Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: huyuting29@163.com. 12. School of Medicine, South China University of Technology, Guangzhou 510006, PR China; Institute of Cell Biology, Zhejiang University, Hangzhou 310058, PR China. Electronic address: lijichen@zju.edu.cn.
Abstract
BACKGROUND: The lack of rapid and efficient diagnostic methods has been one of the most frustrating challenges in controlling the pulmonary tuberculosis (TB) epidemic. This study was aimed to identify novel non-invasive biomarkers for pulmonary TB. METHODS: The subjects in this study were divided into four groups: the pulmonary TB group, the community-acquired pneumonia (CAP) group, the lung cancer (LC) group, and the normal control (NC) group. Plasma small molecule metabolites were investigated in each group by using ultra-high performance liquid chromatography coupled with Q Exactive mass spectrometry. Multivariate statistical methods and bioinformatics were used to analyze the data. RESULTS: We identified three differential plasma metabolites such as, Xanthine, 4-Pyridoxate and d-glutamic acid in the pulmonary TB group, compared to the other groups (CAP, LC and NC). The pathway enrichment analysis indicated that the energy source in pulmonary TB was multi-center, which might be involved in maintaining the reproductive ability and virulence of Mycobacterium tuberculosis. CONCLUSION: The results suggested that Xanthine, 4-Pyridoxate, and d-glutamic acid may serve as potential biomarkers for pulmonary TB. The present study provides experimental basis for developing potential biomarkers of pulmonary TB.
BACKGROUND: The lack of rapid and efficient diagnostic methods has been one of the most frustrating challenges in controlling the pulmonary tuberculosis (TB) epidemic. This study was aimed to identify novel non-invasive biomarkers for pulmonary TB. METHODS: The subjects in this study were divided into four groups: the pulmonary TB group, the community-acquired pneumonia (CAP) group, the lung cancer (LC) group, and the normal control (NC) group. Plasma small molecule metabolites were investigated in each group by using ultra-high performance liquid chromatography coupled with Q Exactive mass spectrometry. Multivariate statistical methods and bioinformatics were used to analyze the data. RESULTS: We identified three differential plasma metabolites such as, Xanthine, 4-Pyridoxate and d-glutamic acid in the pulmonary TB group, compared to the other groups (CAP, LC and NC). The pathway enrichment analysis indicated that the energy source in pulmonary TB was multi-center, which might be involved in maintaining the reproductive ability and virulence of Mycobacterium tuberculosis. CONCLUSION: The results suggested that Xanthine, 4-Pyridoxate, and d-glutamic acid may serve as potential biomarkers for pulmonary TB. The present study provides experimental basis for developing potential biomarkers of pulmonary TB.
Authors: Dongzi Lin; Wei Wang; Feng Qiu; Yumei Li; Xiaolin Yu; Bingyao Lin; Yinwen Chen; Chunyan Lei; Yan Ma; Jincheng Zeng; Jie Zhou Journal: Nan Fang Yi Ke Da Xue Xue Bao Date: 2019-12-30
Authors: Dinesh Kumar Barupal; Priyanka Mahajan; Sadjad Fakouri-Baygi; Robert O Wright; Manish Arora; Susan L Teitelbaum Journal: Environ Int Date: 2022-04-18 Impact factor: 13.352
Authors: R Conde; R Laires; L G Gonçalves; A Rizvi; C Barroso; M Villar; R Macedo; M J Simões; S Gaddam; P Lamosa; L Puchades-Carrasco; A Pineda-Lucena; A B Patel; S C Mande; S Banerjee; M Matzapetakis; A V Coelho Journal: Biomed J Date: 2021-07-24 Impact factor: 7.892