Literature DB >> 31423914

Direct culture-independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses.

Catherine E May1,2, Alan J Guthrie1,2, Martin L Schulman1,2.   

Abstract

We report herein the use of crude extracts obtained from samples of Taylorella equigenitalis-infected horses for the purpose of multi-locus sequence typing (MLST). Samples (n = 36) were collected from horses in South Africa from 1996 to 2017: 34 from genital swabs (stored at -20°C for 2-3 y) and 2 from cryopreserved raw semen aliquots (stored at -70°C for 18 y) prior to assay. The MLST assay showed a single sequence type (ST), designated ST4, that supported a point introduction and thus a common source for the South African outbreak of contagious equine metritis.

Entities:  

Keywords:  contagious equine metritis; direct culture–independent strain typing

Mesh：

Substances：

Year:  2019        PMID： 31423914      PMCID： PMC6727116          DOI： 10.1177/1040638719871089

Source DB:  PubMed          Journal:  J Vet Diagn Invest        ISSN： 1040-6387            Impact factor:   1.279


  17 in total

1.  Genome sequence of Taylorella equigenitalis MCE9, the causative agent of contagious equine metritis.

Authors:  Laurent Hébert; Bouziane Moumen; Fabien Duquesne; Marie-France Breuil; Claire Laugier; Jean-Michel Batto; Pierre Renault; Sandrine Petry
Journal:  J Bacteriol       Date:  2011-01-28       Impact factor: 3.490

2.  Universal primers for fluorescent labelling of PCR fragments--an efficient and cost-effective approach to genotyping by fluorescence.

Authors:  M J Blacket; C Robin; R T Good; S F Lee; A D Miller
Journal:  Mol Ecol Resour       Date:  2012-01-24       Impact factor: 7.090

3.  Development of a real time PCR for the detection of Taylorella equigenitalis directly from genital swabs and discrimination from Taylorella asinigenitalis.

Authors:  P R Wakeley; J Errington; S Hannon; H I J Roest; T Carson; B Hunt; J Sawyer; P Heath
Journal:  Vet Microbiol       Date:  2006-08-14       Impact factor: 3.293

4.  Identification of Taylorella equigenitalis responsible for contagious equine metritis in equine genital swabs by direct polymerase chain reaction.

Authors:  F Duquesne; S Pronost; C Laugier; S Petry
Journal:  Res Vet Sci       Date:  2006-06-27       Impact factor: 2.534

5.  Pulsed-field gel electrophoresis genotyping of Taylorella equigenitalis isolates collected in the United States from 1978 to 2010.

Authors:  Alan M Aalsburg; Matthew M Erdman
Journal:  J Clin Microbiol       Date:  2010-12-29       Impact factor: 5.948

6.  Diagnostic and epidemiologic analysis of the 2008-2010 investigation of a multi-year outbreak of contagious equine metritis in the United States.

Authors:  Matthew M Erdman; Lynn H Creekmore; Patricia E Fox; Angela M Pelzel; Barbara A Porter-Spalding; Alan M Aalsburg; Linda K Cox; Brenda R Morningstar-Shaw; Randall L Crom
Journal:  Prev Vet Med       Date:  2011-06-28       Impact factor: 2.670

Review 7.  Horse species symposium: contagious equine metritis: an insidious threat to the horse breeding industry in the United States.

Authors:  P J Timoney
Journal:  J Anim Sci       Date:  2010-10-01       Impact factor: 3.159

8.  Lateral transmission of equine arteritis virus among Lipizzaner stallions in South Africa.

Authors:  A J Guthrie; P G Howell; J F Hedges; A M Bosman; U B R Balasuriya; W H McCollum; P J Timoney; N J MacLachlan
Journal:  Equine Vet J       Date:  2003-09       Impact factor: 2.888

Review 9.  Recent advances in molecular epidemiology and detection of Taylorella equigenitalis associated with contagious equine metritis (CEM).

Authors:  Motoo Matsuda; John E Moore
Journal:  Vet Microbiol       Date:  2003-12-02       Impact factor: 3.293

10.  Genomic characterization of the Taylorella genus.

Authors:  Laurent Hébert; Bouziane Moumen; Nicolas Pons; Fabien Duquesne; Marie-France Breuil; Didier Goux; Jean-Michel Batto; Claire Laugier; Pierre Renault; Sandrine Petry
Journal:  PLoS One       Date:  2012-01-03       Impact factor: 3.240
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