Literature DB >> 16806331

Identification of Taylorella equigenitalis responsible for contagious equine metritis in equine genital swabs by direct polymerase chain reaction.

F Duquesne1, S Pronost, C Laugier, S Petry.   

Abstract

A direct-PCR assay was developed for the rapid detection of Taylorella equigenitalis, a Gram-negative bacterium responsible for contagious equine metritis (CEM) in Equidae. The bacteria may be detected in equine genital swabs without need for a preliminary step of DNA extraction or bacterial isolation. Specificity was determined with 125 isolates of T. equigenitalis, 24 isolates of Taylorella asinigenitalis, five commensal bacteria of the genital tract and a facultative intracellular pathogen of foals found in large concentration in soil. Our PCR is specific and amplified a 413-bp 16S ribosomal DNA product only in all T. equigenitalis.

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Year:  2006        PMID： 16806331     DOI： 10.1016/j.rvsc.2006.05.001

Source DB:  PubMed          Journal:  Res Vet Sci        ISSN： 0034-5288            Impact factor:   2.534


  8 in total

1.  Genome sequence of Taylorella equigenitalis MCE9, the causative agent of contagious equine metritis.

Authors:  Laurent Hébert; Bouziane Moumen; Fabien Duquesne; Marie-France Breuil; Claire Laugier; Jean-Michel Batto; Pierre Renault; Sandrine Petry
Journal:  J Bacteriol       Date:  2011-01-28       Impact factor: 3.490

2.  Direct culture-independent sequence typing of Taylorella equigenitalis obtained from genital swabs and frozen semen samples from South African horses.

Authors:  Catherine E May; Alan J Guthrie; Martin L Schulman
Journal:  J Vet Diagn Invest       Date:  2019-08-17       Impact factor: 1.279

3.  Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies.

Authors:  Brian W Brooks; Cheryl L Lutze-Wallace; Leann L Maclean; Evgeny Vinogradov; Malcolm B Perry
Journal:  Can J Vet Res       Date:  2010-01       Impact factor: 1.310

4.  Comparison of culture versus quantitative real-time polymerase chain reaction for the detection of Taylorella equigenitalis in field samples from naturally infected horses in Canada and Germany.

Authors:  Susan Nadin-Davis; Margaret K Knowles; Teresa Burke; Reinhard Böse; John Devenish
Journal:  Can J Vet Res       Date:  2015-07       Impact factor: 1.310

5.  Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis.

Authors:  A Tazumi; T Sekizuka; J E Moore; B C Millar; I Taneike; M Matsuda
Journal:  Folia Microbiol (Praha)       Date:  2009-04-18       Impact factor: 2.099

6.  Genomic characterization of the Taylorella genus.

Authors:  Laurent Hébert; Bouziane Moumen; Nicolas Pons; Fabien Duquesne; Marie-France Breuil; Didier Goux; Jean-Michel Batto; Claire Laugier; Pierre Renault; Sandrine Petry
Journal:  PLoS One       Date:  2012-01-03       Impact factor: 3.240

7.  Using the Five Domains Model to Assess the Adverse Impacts of Husbandry, Veterinary, and Equitation Interventions on Horse Welfare.

Authors:  Paul McGreevy; Jeannine Berger; Nic de Brauwere; Orla Doherty; Anna Harrison; Julie Fiedler; Claudia Jones; Sue McDonnell; Andrew McLean; Lindsay Nakonechny; Christine Nicol; Liane Preshaw; Peter Thomson; Vicky Tzioumis; John Webster; Sarah Wolfensohn; James Yeates; Bidda Jones
Journal:  Animals (Basel)       Date:  2018-03-18       Impact factor: 2.752

8.  Comparison of seven nucleic acid amplification tests for detection of Taylorella equigenitalis.

Authors:  Yuta Kinoshita; Hironaga Kakoi; Taichiro Ishige; Takashi Yamanaka; Hidekazu Niwa; Eri Uchida-Fujii; Toshio Nukada; Takanori Ueno
Journal:  J Vet Med Sci       Date:  2021-12-02       Impact factor: 1.267
  8 in total

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