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Literature DB >> 3142355

A crosslinked preparation of E. coli beta-D-galactosidase.

Abstract

beta-D-Galactosidase from E. Coli was crosslinked using glutaraldehyde and two bisimidoesters. With glutaraldehyde and dimethyl adipimidate (DMA), it is possible to obtain preparations having higher activity than the native enzyme. Glutaraldehyde and DMA gave preparations showing enhanced thermal stability. The preparation crosslinked with DMA, when used for continuous hydrolysis of lactose in milk, was found to be significantly better than the native enzyme.

Entities: Chemical Species

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Year: 1987 PMID： 3142355 DOI： 10.1007/bf02798351

Source DB: PubMed Journal: Appl Biochem Biotechnol ISSN： 0273-2289 Impact factor: 2.926

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References

13 in total

1. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

Authors: G R CRAVEN; E STEERS; C B ANFINSEN
Journal: J Biol Chem Date: 1965-06 Impact factor: 5.157

2. Gel entrapped enzymes: kinetic studies of immobilized beta-galactosidase.

Authors: I Hinberg; R Korus; K F O'Driscoll
Journal: Biotechnol Bioeng Date: 1974-07 Impact factor: 4.530

3. Reduction of lactose content of milk by entrapped beta-galactosidase. I. Characteristics of beta-galactosidase from yeast and Escherichia coli.

Authors: F Morisi; M Pastore; A Viglia
Journal: J Dairy Sci Date: 1973-09 Impact factor: 4.034

7. An approach to nearest neighbor analysis of membrane proteins. Application to the human erythrocyte membrane of a method employing cleavable cross-linkages.

Authors: K Wang; F M Richards
Journal: J Biol Chem Date: 1974-12-25 Impact factor: 5.157

A crosslinked preparation of E. coli beta-D-galactosidase.

1. PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12.

2. Gel entrapped enzymes: kinetic studies of immobilized beta-galactosidase.

3. Reduction of lactose content of milk by entrapped beta-galactosidase. I. Characteristics of beta-galactosidase from yeast and Escherichia coli.

4. Characterization and immobilization of E. coli (ATCC-26) Beta-galactosidase.

5. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

6. Determination of free amino groups in proteins by trinitrobenzenesulfonic acid.

7. An approach to nearest neighbor analysis of membrane proteins. Application to the human erythrocyte membrane of a method employing cleavable cross-linkages.

8. The kinetics of beta-galactosidase attached to porous cellulose sheets.

9. Lactose reduction of milk by fiber-entrapped beta-galactosidase. Pilot-plant experiments.

10. Chemical cross-linking of H1 histone to the nucleosomal histones.