| Literature DB >> 31422913 |
Seungmin Han1, Juergen Fink2, David J Jörg3, Eunmin Lee4, Min Kyu Yum1, Lemonia Chatzeli1, Sebastian R Merker5, Manon Josserand6, Teodora Trendafilova6, Amanda Andersson-Rolf2, Catherine Dabrowska6, Hyunki Kim6, Ronald Naumann7, Ji-Hyun Lee8, Nobuo Sasaki9, Richard Lester Mort10, Onur Basak11, Hans Clevers11, Daniel E Stange5, Anna Philpott12, Jong Kyoung Kim13, Benjamin D Simons14, Bon-Kyoung Koo15.
Abstract
The gastric corpus epithelium is the thickest part of the gastrointestinal tract and is rapidly turned over. Several markers have been proposed for gastric corpus stem cells in both isthmus and base regions. However, the identity of isthmus stem cells (IsthSCs) and the interaction between distinct stem cell populations is still under debate. Here, based on unbiased genetic labeling and biophysical modeling, we show that corpus glands are compartmentalized into two independent zones, with slow-cycling stem cells maintaining the base and actively cycling stem cells maintaining the pit-isthmus-neck region through a process of "punctuated" neutral drift dynamics. Independent lineage tracing based on Stmn1 and Ki67 expression confirmed that rapidly cycling IsthSCs maintain the pit-isthmus-neck region. Finally, single-cell RNA sequencing (RNA-seq) analysis is used to define the molecular identity and lineage relationship of a single, cycling, IsthSC population. These observations define the identity and functional behavior of IsthSCs.Entities:
Keywords: Lgr5; Troy; biophysical modeling; deep tissue imaging; gastric corpus isthmus stem cell; intestine; punctuated neutral drift; single-cell RNA-seq; two stem cell compartments; unbiased genetic labeling
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Year: 2019 PMID： 31422913 PMCID： PMC6739486 DOI： 10.1016/j.stem.2019.07.008
Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633