Lin-Chong Su1, Wang-Dong Xu2, Xiao-Yan Liu2, Lu Fu3, An-Fang Huang4. 1. Department of Rheumatology and Immunology, Minda Hospital of Hubei Minzu University, Enshi, China. 2. Department of Evidence-Based Medicine, Southwest Medical University, Luzhou, China. 3. Laboratory Animal Center, Southwest Medical University, Luzhou, China. flfyh@swmu.edu.cn. 4. Department of Rheumatology and Immunology, Affiliated Hospital of Southwest Medical University, Luzhou, China. loutch211@163.com.
Abstract
OBJECTIVES: This study evaluated expression of circRNA in primary Sjögren's syndrome (pSS) patients so as to find novel biomarkers for pSS screening and discussed possible role of circRNA in pSS. We also evaluated expression profile of circRNA in systemic lupus erythematosus (SLE) patients. METHODS: Microarray analysis detected circRNA expression in PBMCs from five paired pSS, SLE patients, and controls. Then, differentially expressed circRNAs were validated in 30 pSS patients as compared to 30 SLE patients, healthy controls. CircRNAs interacting with miRNAs were discussed by Arraystar's homemade miRNA target prediction software. ROC analysis assessed the diagnostic value. RESULTS: We identified 234 differentially expressed circRNAs in pSS patients and verified five selected circRNAs (including hsa_circRNA_001264, hsa_circRNA_104121, hsa_circRNA_045355, hsa_circRNA_103461, hsa_circRNA_105034). Expression of hsa_circRNA_001264, hsa_circRNA_104121, and hsa_circRNA_045355 was strongly related to some clinical, laboratory parameters, and disease activity index in pSS patients. ROC analysis indicated potential diagnostic ability for the three circRNAs in pSS patients. One hundred and forty-eight circRNAs were differently expressed between lupus patients and controls. CONCLUSION: This study provides evidence that hsa_circRNA_001264, hsa_circRNA_104121, and hsa_circRNA_045355 might be biomarkers for pSS, correlate with pSS etiology.Key Points• Many circRNAs were dysregulated in pSS patients.• Differentially expressed circRNAs correlated with pSS clinical, laboratory features.• CircRNAs may be biomarkers for pSS.
OBJECTIVES: This study evaluated expression of circRNA in primary Sjögren's syndrome (pSS) patients so as to find novel biomarkers for pSS screening and discussed possible role of circRNA in pSS. We also evaluated expression profile of circRNA in systemic lupus erythematosus (SLE) patients. METHODS: Microarray analysis detected circRNA expression in PBMCs from five paired pSS, SLEpatients, and controls. Then, differentially expressed circRNAs were validated in 30 pSSpatients as compared to 30 SLEpatients, healthy controls. CircRNAs interacting with miRNAs were discussed by Arraystar's homemade miRNA target prediction software. ROC analysis assessed the diagnostic value. RESULTS: We identified 234 differentially expressed circRNAs in pSSpatients and verified five selected circRNAs (including hsa_circRNA_001264, hsa_circRNA_104121, hsa_circRNA_045355, hsa_circRNA_103461, hsa_circRNA_105034). Expression of hsa_circRNA_001264, hsa_circRNA_104121, and hsa_circRNA_045355 was strongly related to some clinical, laboratory parameters, and disease activity index in pSSpatients. ROC analysis indicated potential diagnostic ability for the three circRNAs in pSSpatients. One hundred and forty-eight circRNAs were differently expressed between lupuspatients and controls. CONCLUSION: This study provides evidence that hsa_circRNA_001264, hsa_circRNA_104121, and hsa_circRNA_045355 might be biomarkers for pSS, correlate with pSS etiology.Key Points• Many circRNAs were dysregulated in pSSpatients.• Differentially expressed circRNAs correlated with pSS clinical, laboratory features.• CircRNAs may be biomarkers for pSS.