Literature DB >> 3141920

Expression of a bovine rhodopsin gene in Xenopus oocytes: demonstration of light-dependent ionic currents.

H G Khorana1, B E Knox, E Nasi, R Swanson, D A Thompson.   

Abstract

Xenopus oocytes express a gene encoding bovine rhodopsin as well as its SP6 RNA polymerase-derived transcripts and total retinal mRNA. The opsin produced is in unglycosylated (30 kDa) and two glycosylated (35 kDa and 41 kDa) forms. Incubation of the cells expressing the above proteins with 11-cis-retinal generates rhodopsin, which was purified by immunoaffinity chromatography. The purified protein shows the expected UV/visible absorption spectrum and characteristic light-dependent activation of the rod outer segment GTP binding protein. Oocytes expressing rhodopsin exhibit light-dependent ionic currents that are detected by voltage-clamp techniques.

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Year:  1988        PMID： 3141920      PMCID： PMC282324          DOI： 10.1073/pnas.85.21.7917

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  33 in total

1.  Synthesis and bleaching of rhodopsin.

Authors:  G WALD; P K BROWN
Journal:  Nature       Date:  1956-01-28       Impact factor: 49.962

2.  A method for preparing stable digitonin solutions for visual pigment extraction.

Authors:  C D Bridges
Journal:  Vision Res       Date:  1977-02       Impact factor: 1.886

3.  Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.

Authors:  A E Sippel
Journal:  Eur J Biochem       Date:  1973-08-01

4.  The molecular basis of visual excitation.

Authors:  G Wald
Journal:  Nature       Date:  1968-08-24       Impact factor: 49.962

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  A transient calcium-dependent chloride current in the immature Xenopus oocyte.

Authors:  M E Barish
Journal:  J Physiol       Date:  1983-09       Impact factor: 5.182

7.  Characterization of bovine rod outer segment G-protein.

Authors:  W Baehr; E A Morita; R J Swanson; M L Applebury
Journal:  J Biol Chem       Date:  1982-06-10       Impact factor: 5.157

8.  Purification and properties of an endo-beta-N-acetylglucosaminidase from Streptomyces griseus.

Authors:  A L Tarentino; F Maley
Journal:  J Biol Chem       Date:  1974-02-10       Impact factor: 5.157

9.  N-methyl-1-deoxynojirimycin, a novel inhibitor of glycoprotein processing, and its effect on fowl plague virus maturation.

Authors:  P A Romero; R Datema; R T Schwarz
Journal:  Virology       Date:  1983-10-15       Impact factor: 3.616

10.  Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors:  J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal:  Biochemistry       Date:  1979-11-27       Impact factor: 3.162
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  29 in total

Review 1.  Use of Xenopus oocytes for the functional expression of plasma membrane proteins.

Authors:  E Sigel
Journal:  J Membr Biol       Date:  1990-09       Impact factor: 1.843

2.  Expression of catfish amino acid taste receptors in Xenopus oocytes.

Authors:  T V Getchell; M Grillo; S S Tate; R Urade; J Teeter; F L Margolis
Journal:  Neurochem Res       Date:  1990-04       Impact factor: 3.996

3.  Light-transduction in melanopsin-expressing photoreceptors of Amphioxus.

Authors:  María del Pilar Gomez; Juan M Angueyra; Enrico Nasi
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-18       Impact factor: 11.205

4.  Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy.

Authors:  S Eskandari; E M Wright; M Kreman; D M Starace; G A Zampighi
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-15       Impact factor: 11.205

5.  Point mutations in bovine opsin can be classified in four groups with respect to their effect on the biosynthetic pathway of opsin.

Authors:  G L DeCaluwé; W J DeGrip
Journal:  Biochem J       Date:  1996-12-15       Impact factor: 3.857

6.  Role of the intradiscal domain in rhodopsin assembly and function.

Authors:  T Doi; R S Molday; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

7.  Altered patterns of N-linked glycosylation of the Torpedo acetylcholine receptor expressed in Xenopus oocytes.

Authors:  A L Buller; M M White
Journal:  J Membr Biol       Date:  1990-05       Impact factor: 1.843

8.  MERTK signaling in the retinal pigment epithelium regulates the tyrosine phosphorylation of GDP dissociation inhibitor alpha from the GDI/CHM family of RAB GTPase effectors.

Authors:  Shameka J Shelby; Kecia L Feathers; Anna M Ganios; Lin Jia; Jason M Miller; Debra A Thompson
Journal:  Exp Eye Res       Date:  2015-08-15       Impact factor: 3.467

9.  Structure and function in rhodopsin: the role of asparagine-linked glycosylation.

Authors:  S Kaushal; K D Ridge; H G Khorana
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

10.  Light-induced currents in Xenopus oocytes expressing bovine rhodopsin.

Authors:  B E Knox; H G Khorana; E Nasi
Journal:  J Physiol       Date:  1993-07       Impact factor: 5.182
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