BACKGROUND: Klebsiella infections are reported from neonatal intensive care units (NICUs) worldwide, but data on their incidence and genetic diversity remain scarce. OBJECTIVE: We determined the incidence and genetic diversity of Klebsiella infections in NICU patients in Rio de Janeiro. METHODS: This was a prospective study including newborns admitted to NICU in three hospitals during April 2005-November 2006 and March 2008-February 2009. Klebsiella pneumoniae isolates were genotyped by multilocus sequence typing (MLST) and extended spectrum β-lactamases (ESBL) were characterized. RESULTS: Klebsiella infections occurred in 38 of 3984 patients (incidence rate, 9.5/1000 admissions); 14 (37%) of these 38 newborns died. Two clonal groups, CC45 and CC1041, caused 11 cases (42% of K. pneumoniae infection). Ten (32%) of the isolates causing infection produced ESBL, 9 of which (83%) carried blaCTX-M-15, all belonging to clonal complex (CC) 45 and CC1041. Nine of these ESBL-producing isolates were confined to only one of the NICUs. MAJOR CONCLUSIONS: The high incidence of Klebsiella infections in NICU in Rio de Janeiro appeared to be due to a combination of frequent sporadic infections caused by multiple K. pneumoniae genotypes and small outbreaks caused by dominant multidrug-resistant clones.
BACKGROUND:Klebsiella infections are reported from neonatal intensive care units (NICUs) worldwide, but data on their incidence and genetic diversity remain scarce. OBJECTIVE: We determined the incidence and genetic diversity of Klebsiella infections in NICU patients in Rio de Janeiro. METHODS: This was a prospective study including newborns admitted to NICU in three hospitals during April 2005-November 2006 and March 2008-February 2009. Klebsiella pneumoniae isolates were genotyped by multilocus sequence typing (MLST) and extended spectrum β-lactamases (ESBL) were characterized. RESULTS:Klebsiella infections occurred in 38 of 3984 patients (incidence rate, 9.5/1000 admissions); 14 (37%) of these 38 newborns died. Two clonal groups, CC45 and CC1041, caused 11 cases (42% of K. pneumoniae infection). Ten (32%) of the isolates causing infection produced ESBL, 9 of which (83%) carried blaCTX-M-15, all belonging to clonal complex (CC) 45 and CC1041. Nine of these ESBL-producing isolates were confined to only one of the NICUs. MAJOR CONCLUSIONS: The high incidence of Klebsiella infections in NICU in Rio de Janeiro appeared to be due to a combination of frequent sporadic infections caused by multiple K. pneumoniae genotypes and small outbreaks caused by dominant multidrug-resistant clones.
Authors: Luke F Chen; Joshua T Freeman; Brad Nicholson; Anna Keiger; Sarah Lancaster; Maria Joyce; Christopher W Woods; Evelyn Cook; Linda Adcock; Susan Louis; Andrea L Cromer; Daniel J Sexton; Deverick J Anderson Journal: Antimicrob Agents Chemother Date: 2013-11-18 Impact factor: 5.191
Authors: Stephen E Mshana; Torsten Hain; Eugen Domann; Eligius F Lyamuya; Trinad Chakraborty; Can Imirzalioglu Journal: BMC Infect Dis Date: 2013-10-07 Impact factor: 3.090
Authors: Liliane Miyuki Seki; Polyana Silva Pereira; Magda de Souza Conceição; Maria José Souza; Elizabeth Andrade Marques; Jupira Miron Carballido; Maria Elisabeth Serqueira de Carvalho; Ana Paula D'Alincourt Carvalho Assef; Marise Dutra Asensi Journal: Braz J Infect Dis Date: 2013-09-20 Impact factor: 3.257