Li Zhang1, Renfang Han2, Xu Zhang2, Gongsi Fang1, Jin Chen1, Jianping Li1, Shengqain Xu3, Long Qian4, Wenjun Chen5, Faming Pan6. 1. Anhui Medical College, 387 Wuhu Road, Hefei, Anhui 230032, China. 2. Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. 3. Department of Rheumatism and Immunity, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China. 4. Department of Rheumatism and Immunity, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601, China. 5. Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. 6. Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China; The Key Laboratory of Major Autoimmune Diseases, Anhui Medical University, 81 Meishan Road, Hefei, Anhui 230032, China. Electronic address: famingpan@ahmu.edu.cn.
Abstract
BACKGROUND: We investigated the characterization of the gut microbiome in Chinese patients with ankylosing spondylitis (AS) and healthy controls (HCs) and to explore the association ofbacteria communities with dietary factors and disease activity. METHODS: 16S ribosomal RNA gene sequencing was performed on fecal DNA isolated from stool samples in consecutive cross-sectional cohorts. Alpha and beta diversities were assessed using QIIME, and comparisons were performed using one-way ANOVA, Student's t-test, and SKN multiple range comparisons to examine differences between groups and a correlation network analysis was performed. RESULTS: We investigated 207 samples from 103 AS patients and 104 HCs. Alpha diversity was not significant difference in AS compared with HCs. For the community structure, Bacteroidetes was the most represented class. Megamonas, Dorea, and Blautia were significantly greater in AS than in HCs, whereas the abundance of Lachnospira, Ruminococcus, and Clostridium_XlVb was significantly lower in AS than in HCs. In addition, Specific gut microbiome was significantly correlated with disease activity and dietary factors. CONCLUSIONS: Our results suggest that the human gut microbiome of AS patients was clearly different from that of HCs and bacteria communities are associated with dietary factors and disease activity.
BACKGROUND: We investigated the characterization of the gut microbiome in Chinese patients with ankylosing spondylitis (AS) and healthy controls (HCs) and to explore the association ofbacteria communities with dietary factors and disease activity. METHODS: 16S ribosomal RNA gene sequencing was performed on fecal DNA isolated from stool samples in consecutive cross-sectional cohorts. Alpha and beta diversities were assessed using QIIME, and comparisons were performed using one-way ANOVA, Student's t-test, and SKN multiple range comparisons to examine differences between groups and a correlation network analysis was performed. RESULTS: We investigated 207 samples from 103 AS patients and 104 HCs. Alpha diversity was not significant difference in AS compared with HCs. For the community structure, Bacteroidetes was the most represented class. Megamonas, Dorea, and Blautia were significantly greater in AS than in HCs, whereas the abundance of Lachnospira, Ruminococcus, and Clostridium_XlVb was significantly lower in AS than in HCs. In addition, Specific gut microbiome was significantly correlated with disease activity and dietary factors. CONCLUSIONS: Our results suggest that the humangut microbiome of AS patients was clearly different from that of HCs and bacteria communities are associated with dietary factors and disease activity.
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