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Literature DB >> 3137577

Coordinate regulation of mRNAs encoding adenosine deaminase, purine nucleoside phosphorylase, and terminal deoxynucleotidyltransferase by phorbol esters in human thymocytes.

Abstract

Incubation of human thymocytes in the presence of phorbol esters caused a reversible decrease in the mRNAs encoding terminal deoxynucleotidyltransferase (TdT; EC 2.7.7.31) and adenosine deaminase (ADA; EC 3.5.4.4) and an increase in the mRNA encoding purine nucleoside phosphorylase (PNP; EC 2.4.2.1). The effect of phorbol esters on TdT and ADA mRNA levels can be attributed to an apparent decrease in the stability of the mRNAs. The changes in ADA, TdT, and PNP mRNAs closely simulate changes in the activities of these enzymes that occur during T-cell differentiation in vivo, suggesting a role for protein kinase C activation in the regulation of the expression of these genes during intrathymic T-cell differentiation. A role for these purine degradation enzymes in the regulation of intracellular pools of the deoxynucleotide substrates of TdT is discussed.

Entities: Chemical Gene Species

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Year: 1988 PMID： 3137577 PMCID： PMC282086 DOI： 10.1073/pnas.85.18.6900

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

24 in total

Coordinate regulation of mRNAs encoding adenosine deaminase, purine nucleoside phosphorylase, and terminal deoxynucleotidyltransferase by phorbol esters in human thymocytes.

1. Adenosine deaminase activity in thymus and other human tissues.

2. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

3. In a radiation chimaera, host H-2 antigens determine immune responsiveness of donor cytotoxic cells.

4. Adenosine-deaminase deficiency in two patients with severely impaired cellular immunity.

5. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

6. Inverse relationship between adenosine deaminase and purine nucleoside phosphorylase in rat lymphocyte populations.

7. Ontogeny of terminal deoxynucleotidyl transferase-positive cells in lymphohemopoietic tissues of rat and mouse.

8. Differences in purine metabolizing enzyme activities in human leukemia T-cell, B-cell and null cell lines.

9. Antagonistic effects of calcium ionophores and phorbol esters on T cell receptor mRNA levels in human thymocytes.

10. On the thymus in the differentiation of "H-2 self-recognition" by T cells: evidence for dual recognition?

1. Point mutations at the purine nucleoside phosphorylase locus impair thymocyte differentiation in the mouse.

2. A potential role for Elf-1 in terminal transferase gene regulation.

3. Intron requirement for expression of the human purine nucleoside phosphorylase gene.

4. Drug-induced death of leukaemic cells after G2/M arrest: higher order DNA fragmentation as an indicator of mechanism.

5. Both LyF-1 and an Ets protein interact with a critical promoter element in the murine terminal transferase gene.

Review 6. Purinergic signalling in endocrine organs.

7. p53 expression is required for thymocyte apoptosis induced by adenosine deaminase deficiency.

8. Terminal deoxynucleotidyl transferase is down-regulated by AP-1-like regulatory elements in human lymphoid cells.