Rubén Cereijo1,2, Siri D Taxerås3, Irene Piquer-Garcia3, Silvia Pellitero3,4, Eva Martínez3, Jordi Tarascó3, Pau Moreno3, José Balibrea5, Manel Puig-Domingo3,4, David Jiménez-Pavón6,7, Carles Lerin8, Francesc Villarroya9,10,11, David Sánchez-Infantes12. 1. Department of Biochemistry and Molecular Biomedicine, Institute of Biomedicine, University of Barcelona, Barcelona, Catalonia, Spain. 2. Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Barcelona, Catalonia, Spain. 3. Germans Trias i Pujol Research Institute, Campus Can Ruti, Carretera de Can Ruti, Camí de les Escoles s/n, Badalona, 08916, Barcelona, Spain. 4. Biomedical Research Center (Red Fisiopatología de la Diabetes y enfermedades metabólicas) (CIBERDEM), ISCIII, Madrid, Spain. 5. Metabolic and Bariatric Surgery Unit, EAC-BS Center of Excellence, Vall d'Hebron University Hospital, Barcelona, Spain. 6. MOVE-IT Research group and Department of Physical Education, Faculty of Education Sciences, University of Cádiz, Cádiz, Spain. 7. Institute of Research and Innovation in Biomedical Sciences of the Province of Cádiz (INiBICA), University of Cádiz, Cádiz, Spain. 8. Institut de Recerca Sant Joan de Déu, Barcelona, Catalonia, Spain. 9. Department of Biochemistry and Molecular Biomedicine, Institute of Biomedicine, University of Barcelona, Barcelona, Catalonia, Spain. fvillarroya@ub.edu. 10. Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Barcelona, Catalonia, Spain. fvillarroya@ub.edu. 11. Institut de Recerca Sant Joan de Déu, Barcelona, Catalonia, Spain. fvillarroya@ub.edu. 12. Germans Trias i Pujol Research Institute, Campus Can Ruti, Carretera de Can Ruti, Camí de les Escoles s/n, Badalona, 08916, Barcelona, Spain. dsanchez@igtp.cat.
Abstract
INTRODUCTION: miRNAs are small non-coding RNAs, some of which are expressed in adipose tissues, are present in the circulation, and are regulated in obesity. Bariatric surgery (BS) has been proposed to lead to activation of brown adipose tissue, an effect that may be related to beneficial effects of BS on systemic metabolism. Here, we evaluated circulating levels of miR-92a and miR-99b, two miRNAs proposed as biomarkers of brown fat activity, in a cohort of patients with severe obesity before and after BS, and studied their potential relationship with BS-associated improvements in metabolic parameters. METHODS: Circulating levels of miR-92a and miR-99b were quantified in a cohort of 26 patients (age, 48 ± 10 years; BMI, 45 ± 7 kg/m2) before and 6 months after BS. Clinical parameters were determined at different time points and correlations among them were studied. RESULTS: Basal levels of miR-92a were significantly increased in patients with obesity relative to lean controls. Serum miR-92a levels were strongly reduced at 6 months after BS, reaching levels similar to those in controls. Serum miR-99b levels were unchanged in relation to both the obese condition and BS. Elevated levels of miR-92a were directly correlated with worsened glucose homeostasis parameters and poor BS outcome. CONCLUSIONS: Our findings show that miR-92a is elevated in conditions of obesity, and its reduction after BS correlates with metabolic improvement. Further studies would be necessary to establish miR-92a as serum biomarker and potential predictor of the BS success in improving the metabolic status of patients with obesity.
INTRODUCTION: miRNAs are small non-coding RNAs, some of which are expressed in adipose tissues, are present in the circulation, and are regulated in obesity. Bariatric surgery (BS) has been proposed to lead to activation of brown adipose tissue, an effect that may be related to beneficial effects of BS on systemic metabolism. Here, we evaluated circulating levels of miR-92a and miR-99b, two miRNAs proposed as biomarkers of brown fat activity, in a cohort of patients with severe obesity before and after BS, and studied their potential relationship with BS-associated improvements in metabolic parameters. METHODS: Circulating levels of miR-92a and miR-99b were quantified in a cohort of 26 patients (age, 48 ± 10 years; BMI, 45 ± 7 kg/m2) before and 6 months after BS. Clinical parameters were determined at different time points and correlations among them were studied. RESULTS: Basal levels of miR-92a were significantly increased in patients with obesity relative to lean controls. Serum miR-92a levels were strongly reduced at 6 months after BS, reaching levels similar to those in controls. Serum miR-99b levels were unchanged in relation to both the obese condition and BS. Elevated levels of miR-92a were directly correlated with worsened glucose homeostasis parameters and poor BS outcome. CONCLUSIONS: Our findings show that miR-92a is elevated in conditions of obesity, and its reduction after BS correlates with metabolic improvement. Further studies would be necessary to establish miR-92a as serum biomarker and potential predictor of the BS success in improving the metabolic status of patients with obesity.
Entities:
Keywords:
Bariatric surgery; Brown adipose tissue; Obesity; miR-92a; miR-99b
Authors: G H E J Vijgen; N D Bouvy; G J J Teule; B Brans; J Hoeks; P Schrauwen; W D van Marken Lichtenbelt Journal: J Clin Endocrinol Metab Date: 2012-04-24 Impact factor: 5.958
Authors: Wouter D van Marken Lichtenbelt; Joost W Vanhommerig; Nanda M Smulders; Jamie M A F L Drossaerts; Gerrit J Kemerink; Nicole D Bouvy; Patrick Schrauwen; G J Jaap Teule Journal: N Engl J Med Date: 2009-04-09 Impact factor: 91.245
Authors: Aaron M Cypess; Sanaz Lehman; Gethin Williams; Ilan Tal; Dean Rodman; Allison B Goldfine; Frank C Kuo; Edwin L Palmer; Yu-Hua Tseng; Alessandro Doria; Gerald M Kolodny; C Ronald Kahn Journal: N Engl J Med Date: 2009-04-09 Impact factor: 91.245
Authors: Claudine Seeliger; Tanja Krauss; Julius Honecker; Laura Aline Mengel; Lise Buekens; Alberto Mesas-Fernández; Thomas Skurk; Melina Claussnitzer; Hans Hauner Journal: Sci Rep Date: 2022-06-10 Impact factor: 4.996