Literature DB >> 313397

A morphometrical study of the exocrine pancreatic cell in fasted and fed frogs.

J W Slot, J J Geuze.   

Abstract

The influence of feeding on the ultrastruct of the frog exocrine pancreatic cell was studied by morphometrical procedures. Volume and surface of various cell structures were measured and expressed per unit cell volume. The average cellular size was not influenced by feeding. Though protein synthesis changes 5-to 10-fold (van Venrooij, W. J., and C. Poort. 1971. Biochim. Biophys. Acta. 247:468-470), no significant differences were observed in the amount of membrane that constitutes the rough endoplasmic reticulum (RER) and that represented the major part of total cellular membranes. The appearance of the RER changed. When fasted, most of its membrane was arranged in stacks of tightly packed, narrow cisternae. Within 4 h after feeding, these cisternae were separated and irregularly dilated, and ribosomes became ordered in typical rosettes on their surface. The total volume of the Golgi system increased twofold after feeding. The vesicular and tubular elements at the Golgi periphery did not change, but the volumes of the Golgi cisternae and the condensing vacuoles increased 2.5- and 6-fold, respectively. The increased in the amount of membrane present in these structures was only 1.6- and 3.5-fold, which reflects the more distended appearance of the cisternae and the rounded shape of the condensing vacuoles after feeding. Feeding halved the number of secretory granules per cell, and signs of exocytosis were more common than in fasted animals. These findings suggest that, in the frog pancreatic cell, fluctuations in the production of secretory proteins are not accompanied by an important breakdown and renewal of cellular membranes. This may favor a rapid and strong response of the cell to feeding.

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Year:  1979        PMID: 313397      PMCID: PMC2110376          DOI: 10.1083/jcb.80.3.692

Source DB:  PubMed          Journal:  J Cell Biol        ISSN: 0021-9525            Impact factor:   10.539


  9 in total

1.  Synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (Rana esculenta). II. An in vitro study of the transport process and the influence of temperature.

Authors:  J W Slot; J J Geuze
Journal:  Cell Tissue Res       Date:  1976-03-16       Impact factor: 5.249

2.  OSMIUM IMPREGNATION OF THE GOLGI APPARATUS.

Authors:  D S FRIEND; M J MURRAY
Journal:  Am J Anat       Date:  1965-07

3.  Synthesis and intracellular transport of proteins in the exocrine pancreas of the frog (Rana esculenta). I. An ultrastructural and autoradiographic study.

Authors:  J W Slot; J J Geuze; C Poort
Journal:  Cell Tissue Res       Date:  1974       Impact factor: 5.249

4.  Synthesis and transfer of amylase in pigeon pancreatic micromosomes.

Authors:  C M Redman; P Siekevitz; G E Palade
Journal:  J Biol Chem       Date:  1966-03-10       Impact factor: 5.157

5.  Studies of the secretory process in the mammalian exocrine pancreas. I. The condensing vacuoles.

Authors:  A B Novikoff; M Mori; N Quintana; A Yam
Journal:  J Cell Biol       Date:  1977-10       Impact factor: 10.539

6.  An ultrastructural study of the pancreatic acinar cell in mitosis, with special reference to changes in the Golgi complex.

Authors:  R N Melmed; C J Benitez; S J Holt
Journal:  J Cell Sci       Date:  1973-01       Impact factor: 5.285

7.  Synthesis, intracellular transport, and discharge of secretory proteins in stimulated pancreatic exocrine cells.

Authors:  J D Jamieson; G E Palade
Journal:  J Cell Biol       Date:  1971-07       Impact factor: 10.539

8.  Regulation of protein synthesis in the venom gland of viperid snakes.

Authors:  U Oron; A Bdolah
Journal:  J Cell Biol       Date:  1973-01       Impact factor: 10.539

9.  Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas.

Authors:  J W Slot; G J Strous; J J Geuze
Journal:  J Cell Biol       Date:  1979-03       Impact factor: 10.539

  9 in total
  7 in total

1.  Cytoplasmic granule formation in mouse pancreatic acinar cells. Evidence for formation of immature granules (condensing vacuoles) by aggregation and fusion of progranules of unit size, and for reductions in membrane surface area and immature granule volume during granule maturation.

Authors:  S Lew; I Hammel; S J Galli
Journal:  Cell Tissue Res       Date:  1994-11       Impact factor: 5.249

2.  Freeze-fracture study on the whorls of rough endoplasmic reticulum in the exocrine pancreatic cells of the Japanese newt and African clawed toad.

Authors:  K Taira; H Mutoh; S Shibasaki
Journal:  Cell Tissue Res       Date:  1981       Impact factor: 5.249

3.  A Phos-tag-based approach reveals the extent of physiological endoplasmic reticulum stress.

Authors:  Liu Yang; Zhen Xue; Yin He; Shengyi Sun; Hui Chen; Ling Qi
Journal:  PLoS One       Date:  2010-07-16       Impact factor: 3.240

4.  A morphometric study of developing pancreatic acinar cells of rats during prenatal life.

Authors:  Y Uchiyama; M Watanabe
Journal:  Cell Tissue Res       Date:  1984       Impact factor: 5.249

5.  A morphometric study of 24-hour variations in subcellular structures of the rat pancreatic acinar cell.

Authors:  Y Uchiyama; K Saito
Journal:  Cell Tissue Res       Date:  1982       Impact factor: 5.249

6.  Beta-COP localizes mainly to the cis-Golgi side in exocrine pancreas.

Authors:  A Oprins; R Duden; T E Kreis; H J Geuze; J W Slot
Journal:  J Cell Biol       Date:  1993-04       Impact factor: 10.539

7.  Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas.

Authors:  J W Slot; G J Strous; J J Geuze
Journal:  J Cell Biol       Date:  1979-03       Impact factor: 10.539

  7 in total

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