Mohamed A Hassan1, Tamer M Tamer2, Asmaa A Rageh2, Alaa M Abou-Zeid3, Eman H F Abd El-Zaher3, El-Refaie Kenawy4. 1. Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box 21934, Alexandria, Egypt. Electronic address: madel@srtacity.sci.eg. 2. Polymer Materials Research Department, Advanced Technologies, and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City, P.O. Box 21934, Alexandria, Egypt. 3. Department of Botany, Faculty of Science, Tanta University, Tanta, Egypt. 4. Chemistry Department, Polymer Research Group Faculty of Science, Tanta University, Tanta, Egypt.
Abstract
AIM: This study was undertaken to inspect the preponderance of multidrug-resistant (MDR) microorganisms in microbial infected diabetic foot ulcers (DFUs) in north Egypt. Moreover, their later impact on the patients and previous antibiotic therapies were taken into consideration. METHODS: To accomplish this goal, twenty-two of diabetic foot patients with purulent wounds were enrolled in this prospective study. These wounds were swabbed and the antibiotics susceptibility patterns of most virulent bacteria and yeast were studied. Furthermore, bacterial and yeast strains were identified using 16S rRNA and 16S rRNA nucleotide sequences, respectively, and following their phenotypic characteristics employing the VITEK 2 system. RESULTS: Microbial profiles showed a predominance of monomicrobial infections (77.3%), while polymicrobial infections were found in 22.7%. A total of 24 bacterial isolates (15 Gram-positive and 9 Gram-negative) and four yeast isolates were perceived. Four bacteria were selected based on their resistance toward more than six of empirical antibiotics. They were identified and deposited in GenBank as Acinetobacter baumanni MT3 (KY421195), Staphylococcus aureus MT1 (KY421197), Klebsiella pneumonia MT2 (KY421196), and Staphylococcus aureus MT4 (KY421198). On the other hand, one strain belonged to yeast was opted and identified as Candida albicans MT5 (MG851796). CONCLUSION: These findings might effectively help to avert the severe complications of diabetic foot infections (DFIs) besides our endeavours to find new antimicrobial wound dressings.
AIM: This study was undertaken to inspect the preponderance of multidrug-resistant (MDR) microorganisms in microbial infected diabetic foot ulcers (DFUs) in north Egypt. Moreover, their later impact on the patients and previous antibiotic therapies were taken into consideration. METHODS: To accomplish this goal, twenty-two of diabetic footpatients with purulent wounds were enrolled in this prospective study. These wounds were swabbed and the antibiotics susceptibility patterns of most virulent bacteria and yeast were studied. Furthermore, bacterial and yeast strains were identified using 16S rRNA and 16S rRNA nucleotide sequences, respectively, and following their phenotypic characteristics employing the VITEK 2 system. RESULTS: Microbial profiles showed a predominance of monomicrobial infections (77.3%), while polymicrobial infections were found in 22.7%. A total of 24 bacterial isolates (15 Gram-positive and 9 Gram-negative) and four yeast isolates were perceived. Four bacteria were selected based on their resistance toward more than six of empirical antibiotics. They were identified and deposited in GenBank as Acinetobacter baumanni MT3 (KY421195), Staphylococcus aureus MT1 (KY421197), Klebsiella pneumonia MT2 (KY421196), and Staphylococcus aureus MT4 (KY421198). On the other hand, one strain belonged to yeast was opted and identified as Candida albicans MT5 (MG851796). CONCLUSION: These findings might effectively help to avert the severe complications of diabetic foot infections (DFIs) besides our endeavours to find new antimicrobial wound dressings.
Authors: Fakhria A Al-Joufi; Khalid M Aljarallah; Soheir A Hagras; Ibrahim M Al Hosiny; Mounir M Salem-Bekhit; Abdullah M E Youssof; Faiyaz Shakeel Journal: 3 Biotech Date: 2020-06-30 Impact factor: 2.406
Authors: Mateusz Bogacz; Tadeusz Morawiec; Joanna Śmieszek-Wilczewska; Katarzyna Janowska-Bogacz; Anna Bubiłek-Bogacz; Rafał Rój; Karolina Pinocy; Anna Mertas Journal: Biomed Res Int Date: 2019-10-07 Impact factor: 3.246