| Literature DB >> 31311154 |
Paul Dowling1,2, Margit Zweyer3, Dieter Swandulla3, Kay Ohlendieck4,5.
Abstract
The authors wish to make the following correction to their paper [...].Entities:
Year: 2019 PMID: 31311154 PMCID: PMC6789670 DOI: 10.3390/proteomes7030028
Source DB: PubMed Journal: Proteomes ISSN: 2227-7382
Figure 2Overview of the bioanalytical advantages versus potential technical limitations of gel-based top-down proteomics, especially in relation to two-dimensional gel electrophoresis (2DGE) approaches.
Figure 5Overview of fluorescence two-dimensional difference in-gel electrophoresis (2D-DIGE) techniques for the comparative analysis of proteoforms. The upper panel shows the two main DIGE approaches for the systematic pre-electrophoretic labelling of protein fractions using fluorescent CyDyes. These methods utilise minimal sub-stochiometric labelling of assessable lysines in proteins or saturation labelling of assessable cysteines in proteins. The lower panel outlines three-dye (Cy2, Cy3, Cy5) versus two-dye (Cy3, Cy5) DIGE labelling methodology.