Literature DB >> 33377090

Protocol for the Bottom-Up Proteomic Analysis of Mouse Spleen.

Paul Dowling1,2, Stephen Gargan1,2, Margit Zweyer3, Michael Henry4, Paula Meleady4, Dieter Swandulla5, Kay Ohlendieck1,2.   

Abstract

This protocol describes the comparative proteomic profiling of the spleen of wild type versus mdx-4cv mouse, a model of dystrophinopathy. We detail sample preparation for bottom-up proteomic mass spectrometry experiments, including homogenization of tissue, protein concentration measurements, protein digestion, and removal of interfering chemicals. We then describe the steps for mass spectrometric analysis and bioinformatic evaluation. For complete details on the use and execution of this protocol, please refer to Dowling et al. (2020).
© 2020 The Author(s).

Entities:  

Keywords:  High Throughput Screening; Mass Spectrometry; Model Organisms; Protein Biochemistry; Proteomics

Mesh：

Substances：

Year:  2020        PMID： 33377090      PMCID： PMC7757555          DOI： 10.1016/j.xpro.2020.100196

Source DB:  PubMed          Journal:  STAR Protoc        ISSN： 2666-1667


  29 in total

1.  Proteomic analysis of the sarcolemma-enriched fraction from dystrophic mdx-4cv skeletal muscle.

Authors:  Sandra Murphy; Margit Zweyer; Michael Henry; Paula Meleady; Rustam R Mundegar; Dieter Swandulla; Kay Ohlendieck
Journal:  J Proteomics       Date:  2018-02-02       Impact factor: 4.044

Review 2.  AIM/CD5L: a key protein in the control of immune homeostasis and inflammatory disease.

Authors:  Lucía Sanjurjo; Gemma Aran; Nerea Roher; Annabel F Valledor; Maria-Rosa Sarrias
Journal:  J Leukoc Biol       Date:  2015-06-05       Impact factor: 4.962

3.  Recovery of induced mutations for X chromosome-linked muscular dystrophy in mice.

Authors:  V M Chapman; D R Miller; D Armstrong; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

Review 4.  Update in Duchenne and Becker muscular dystrophy.

Authors:  Megan A Waldrop; Kevin M Flanigan
Journal:  Curr Opin Neurol       Date:  2019-10       Impact factor: 5.710

5.  Multiplexed MRM-based assays for the quantitation of proteins in mouse plasma and heart tissue.

Authors:  Andrew J Percy; Sarah A Michaud; Armando Jardim; Nicholas J Sinclair; Suping Zhang; Yassene Mohammed; Andrea L Palmer; Darryl B Hardie; Juncong Yang; Andre M LeBlanc; Christoph H Borchers
Journal:  Proteomics       Date:  2016-11-18       Impact factor: 3.984

Review 6.  Duchenne and Becker Muscular Dystrophies: A Review of Animal Models, Clinical End Points, and Biomarker Quantification.

Authors:  Kristin Wilson; Crystal Faelan; Janet C Patterson-Kane; Daniel G Rudmann; Steven A Moore; Diane Frank; Jay Charleston; Jon Tinsley; G David Young; Anthony J Milici
Journal:  Toxicol Pathol       Date:  2017-10-03       Impact factor: 1.902

Review 7.  Computational methods in mass spectrometry-based structural proteomics for studying protein structure, dynamics, and interactions.

Authors:  Seungjin Na; Eunok Paek
Journal:  Comput Struct Biotechnol J       Date:  2020-06-10       Impact factor: 7.271

Review 8.  A Critical Review of Bottom-Up Proteomics: The Good, the Bad, and the Future of this Field.

Authors:  Emmalyn J Dupree; Madhuri Jayathirtha; Hannah Yorkey; Marius Mihasan; Brindusa Alina Petre; Costel C Darie
Journal:  Proteomes       Date:  2020-07-06

9.  Mouse models of neutropenia reveal progenitor-stage-specific defects.

Authors:  David E Muench; Andre Olsson; Kyle Ferchen; Giang Pham; Rachel A Serafin; Somchai Chutipongtanate; Pankaj Dwivedi; Baobao Song; Stuart Hay; Kashish Chetal; Lisa R Trump-Durbin; Jayati Mookerjee-Basu; Kejian Zhang; Jennifer C Yu; Carolyn Lutzko; Kasiani C Myers; Kristopher L Nazor; Kenneth D Greis; Dietmar J Kappes; Sing Sing Way; Nathan Salomonis; H Leighton Grimes
Journal:  Nature       Date:  2020-04-22       Impact factor: 49.962
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  4 in total

Review 1.  Histopathology of Duchenne muscular dystrophy in correlation with changes in proteomic biomarkers.

Authors:  Margit Zweyer; Hemmen Sabir; Paul Dowling; Stephen Gargan; Sandra Murphy; Dieter Swandulla; Kay Ohlendieck
Journal:  Histol Histopathol       Date:  2021-12-07       Impact factor: 2.303

2.  Proteomic profiling of the interface between the stomach wall and the pancreas in dystrophinopathy.

Authors:  Paul Dowling; Stephen Gargan; Margit Zweyer; Hemmen Sabir; Michael Henry; Paula Meleady; Dieter Swandulla; Kay Ohlendieck
Journal:  Eur J Transl Myol       Date:  2021-03-26

3.  Mass Spectrometric Profiling of Extraocular Muscle and Proteomic Adaptations in the mdx-4cv Model of Duchenne Muscular Dystrophy.

Authors:  Stephen Gargan; Paul Dowling; Margit Zweyer; Jens Reimann; Michael Henry; Paula Meleady; Dieter Swandulla; Kay Ohlendieck
Journal:  Life (Basel)       Date:  2021-06-22

4.  Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis.

Authors:  Gaurav Tripathi; Nupur Sharma; Vasundhra Bindal; Manisha Yadav; Babu Mathew; Shvetank Sharma; Ekta Gupta; Jaswinder Singh Maras; Shiv Kumar Sarin
Journal:  STAR Protoc       Date:  2021-11-27
  4 in total

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