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Literature DB >> 3131026

Transformation of Penicillium chrysogenum using the Aspergillus nidulans amdS gene as a dominant selective marker.

Abstract

The Aspergillus nidulans acetamidase gene (amdS) has been used to transform Penicillium chrysogenum at low frequency. Several transformants were tested and shown to be mitotically stable. Southern blot analysis indicated that transforming DNA had integrated into the chromosomal DNA, possibly at multiple sites.

Entities: Species

Mesh：

Year: 1987 PMID： 3131026 DOI： 10.1007/bf00393928

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

8 in total

1. Nuclear and extranuclear inheritance of oligomycin resistance in Aspergillus nidulans.

Authors: R T Rowlands; G Turner
Journal: Mol Gen Genet Date: 1973-11-12

2. Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations.

Authors: M J Hynes; C M Corrick; J A King
Journal: Mol Cell Biol Date: 1983-08 Impact factor: 4.272

3. Development of a high-frequency transforming vector for Aspergillus nidulans.

Authors: D J Ballance; G Turner
Journal: Gene Date: 1985 Impact factor: 3.688

4. Transformation of Aspergillus nidulans by using a trpC plasmid.

Authors: M M Yelton; J E Hamer; W E Timberlake
Journal: Proc Natl Acad Sci U S A Date: 1984-03 Impact factor: 11.205

5. Transformation by integration in Aspergillus nidulans.

Authors: J Tilburn; C Scazzocchio; G G Taylor; J H Zabicky-Zissman; R A Lockington; R W Davies
Journal: Gene Date: 1983-12 Impact factor: 3.688

6. Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA.

Authors: M E Case; M Schweizer; S R Kushner; N H Giles
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

7. Transformation of Aspergillus nidulans by the orotidine-5'-phosphate decarboxylase gene of Neurospora crassa.

Authors: D J Ballance; F P Buxton; G Turner
Journal: Biochem Biophys Res Commun Date: 1983-04-15 Impact factor: 3.575

8. Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

Authors: J M Kelly; M J Hynes
Journal: EMBO J Date: 1985-02 Impact factor: 11.598

8 in total

18 in total

1. The genetic stability of Penicillium chrysogenum transformants in a fermentor.

Authors: D V Renno; G Saunders; A T Bull; G Holt
Journal: Appl Microbiol Biotechnol Date: 1990-12 Impact factor: 4.813

Review 2. Molecular biology of penicillin and cephalosporin biosynthesis.

Authors: S W Queener
Journal: Antimicrob Agents Chemother Date: 1990-06 Impact factor: 5.191

3. Two Penicillium camembertii mutants affected in the production of cyclopiazonic acid.

Authors: R Geisen; E Glenn; L Leistner
Journal: Appl Environ Microbiol Date: 1990-11 Impact factor: 4.792

4. Amplification of the isopenicillin N synthetase gene in a strain of Penicillium chrysogenum producing high levels of penicillin.

Authors: D J Smith; J H Bull; J Edwards; G Turner
Journal: Mol Gen Genet Date: 1989-04

5. Expression of the Aspergillus niger glucose oxidase gene in Penicillium nalgiovense.

Authors: R Geisen
Journal: World J Microbiol Biotechnol Date: 1995-05 Impact factor: 3.312

6. Cloning and expression of a hybrid Streptomyces clavuligerus cefE gene in Penicillium chrysogenum.

Authors: C A Cantwell; R J Beckmann; J E Dotzlaf; D L Fisher; P L Skatrud; W K Yeh; S W Queener
Journal: Curr Genet Date: 1990-03 Impact factor: 3.886

7. Two genes involved in penicillin biosynthesis are linked in a 5.1 kb SalI fragment in the genome of Penicillium chrysogenum.

Authors: B Díez; J L Barredo; E Alvarez; J M Cantoral; P van Solingen; M A Groenen; A E Veenstra; J F Martín
Journal: Mol Gen Genet Date: 1989-09

8. Large amplification of a 35-kb DNA fragment carrying two penicillin biosynthetic genes in high penicillin producing strains of Penicillium chrysogenum.

Authors: J L Barredo; B Díez; E Alvarez; J F Martín
Journal: Curr Genet Date: 1989-12 Impact factor: 3.886

9. Transformation of Penicillium chrysogenum with a dominant selectable marker.

Authors: J H Bull; D J Smith; G Turner
Journal: Curr Genet Date: 1988-05 Impact factor: 3.886

10. Development of a new transformant selection system for Penicillium chrysogenum: isolation and characterization of the P. chrysogenum acetyl-coenzyme A synthetase gene (facA) and its use as a homologous selection marker.

Authors: R J Gouka; W van Hartingsveldt; R A Bovenberg; C M van Zeijl; C A van den Hondel; R F van Gorcom
Journal: Appl Microbiol Biotechnol Date: 1993-01 Impact factor: 4.813