Yaoyong Lu1, Xubin Deng2, Guanghui Xiao3, Xin Zheng4, Lei Ma2, Wendong Huang5. 1. Department of Oncology (Section 3), Gaozhou People's Hospital, Gaozhou, Guangdong, PR China. 2. Department of internal medicine, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou, PR China. 3. Department of Cancer Biology Program, Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, PA 215, USA. 4. Department of internal medicine, Yanling Hospital of Southern Medical University, Guangzhou, PR China. 5. Department of Pharmacy, Maoming People's Hospital, Maoming, Guangdong, PR China.
Abstract
Aim: To study the role of circRNA (circ_0001730) in glioblastoma. Materials & methods: The interaction between circ_0001730 and miR-326 was confirmed by FISH, RNA pull down, RNA-binding protein immunoprecipitation and luciferase reporter assays. Cell proliferation and growth were determined by MTT, EdU and colony formation assays. Cell migration was assessed by the Boyden assay. Results: The levels of circ_0001730 were elevated in glioblastoma cell lines and tissues. circ_0001730 downregulation suppressed migration and proliferation in glioblastoma cells. SP1 bounds to the promoter of circ_0001730 host gene EPHB4 thereby increasing the expression of circ_0001730. circ_0001730 activated the Wnt/β-catenin pathway via the miR-326/Wnt7B axis. Conclusion: circ_000173 promoted growth and invasion in glioblastoma cells via the miR-326/Wnt7B axis.
Aim: To study the role of circRNA (circ_0001730) in glioblastoma. Materials & methods: The interaction between circ_0001730 and miR-326 was confirmed by FISH, RNA pull down, RNA-binding protein immunoprecipitation and luciferase reporter assays. Cell proliferation and growth were determined by MTT, EdU and colony formation assays. Cell migration was assessed by the Boyden assay. Results: The levels of circ_0001730 were elevated in glioblastoma cell lines and tissues. circ_0001730 downregulation suppressed migration and proliferation in glioblastoma cells. SP1 bounds to the promoter of circ_0001730 host gene EPHB4 thereby increasing the expression of circ_0001730. circ_0001730 activated the Wnt/β-catenin pathway via the miR-326/Wnt7B axis. Conclusion: circ_000173 promoted growth and invasion in glioblastoma cells via the miR-326/Wnt7B axis.
Entities:
Keywords:
Wnt7B; circRNAs; epithelial to mesenchymal transition; glioblastoma; miR-326; β-catenin