Biotransformation of progesterone and testosterone enanthate by Circinella muscae.

In this study, the biotransformation of progesterone (1) and testosterone enanthate (5) using the whole cells of Circinella muscae was investigated for the first time. Microbial transformation of 1 with C. muscae afforded three known metabolites including 9α-hydroxyprogesterone (2), 14α-hydroxyprogesterone (3) and 6β,14α dihydroxyprogesterone (4) after 6 days of incubation at 26 °C. The biotransformation of 5 with C. muscae yielded a new metabolite; 8β,14α-dihydroxytestosterone (8), in addition to two known metabolites; 6β-hydroxytestosterone (6), and 9α-hydroxytestosterone (7). The structure of the metabolites were established on the basis of spectroscopic data.