| Literature DB >> 31295427 |
Alexandra M Bender1, Francesco R Simonetti1, Mithra R Kumar1, Emily J Fray1, Katherine M Bruner1, Andrew E Timmons1, Katherine Y Tai1, Katharine M Jenike1, Annukka A R Antar1, Po-Ting Liu2, Ya-Chi Ho1, Dana N Raugi3, Moussa Seydi4, Geoffrey S Gottlieb3, Afam A Okoye5, Gregory Q Del Prete6, Louis J Picker5, Joseph L Mankowski7, Jeffrey D Lifson6, Janet D Siliciano1, Greg M Laird8, Dan H Barouch2, Janice E Clements7, Robert F Siliciano9.
Abstract
Evaluation of HIV cure strategies is complicated by defective proviruses that persist in ART-treated patients but are irrelevant to cure. Non-human primates (NHP) are essential for testing cure strategies. However, the persisting proviral landscape in ART-treated NHPs is uncharacterized. Here, we describe viral genomes persisting in ART-treated, simian immunodeficiency virus (SIV)-infected NHPs, simian-human immunodeficiency virus (SHIV)-infected NHPs, and humans infected with HIV-2, an SIV-related virus. The landscapes of persisting SIV, SHIV, and HIV-2 genomes are also dominated by defective sequences. However, there was a significantly higher fraction of intact SIV proviral genomes compared to ART-treated HIV-1 or HIV-2 infected humans. Compared to humans with HIV-1, SIV-infected NHPs had more hypermutated genomes, a relative paucity of clonal SIV sequences, and a lower frequency of deleted genomes. Finally, we report an assay for measuring intact SIV genomes which may have value in cure research.Entities:
Keywords: HIV-2; SHIV; SIV; clonal expansion; defective provirus; latent reservoir
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Year: 2019 PMID： 31295427 PMCID： PMC6724192 DOI： 10.1016/j.chom.2019.06.005
Source DB: PubMed Journal: Cell Host Microbe ISSN： 1931-3128 Impact factor: 31.316